器官移植2026，Vol.17Issue(3)：405-412,8.DOI:10.12464/j.issn.1674-7445.2026053
免疫复合物-血小板FcγRⅡa(CD32a)介导的异种移植相关凝血功能障碍机制的初步研究
A preliminary study on the mechanism of xenotransplantation-related coagulation dysfunction mediated by immune complexes-platelet FcγRⅡa(CD32a)
摘要
Abstract
Objective To establish an"human serum-porcine aortic endothelial cells(PAEC)-human platelets"in vitro model and explore the mechanism of xenotransplantation-related coagulation dysfunction mediated by immune complexes-platelet FcγR Ⅱ a(CD32a)receptor.Methods Healthy human serum was co-incubated with PAEC to prepare the supernatant containing immune complexes,which was then used to stimulate healthy human platelets,or directly treated with the serum of xenogeneic liver transplant recipients.Flow cytometry was used to detect platelet activation markers CD62P and surface IgG binding levels,and the platelet adhesion function was evaluated by platelet-PAEC adhesion experiments.CD32a blocking antibody Ⅳ.3 and SYK blocker SKYIN 4 were used to clarify the signaling pathways.Results The supernatant from the co-incubation of healthy human serum and PAEC could significantly induce platelet activation and endothelial adhesion.The use of the serum from xenogeneic liver transplant recipients could also significantly induce platelet activation.Antibody Ⅳ.3 and SYK blocker SKYIN 4 could significantly inhibit these effects.Conclusions In xenotransplantation,the immune complexes formed by human serum antibodies and porcine endothelial antigens may induce abnormal platelet activation through the platelet CD32a receptor,which is an important mechanism of non-complement-dependent post-transplant coagulation dysfunction,providing a new target for the intervention of coagulation complications in xenotransplantation.关键词
异种移植/凝血功能障碍/血小板/FcγRⅡa(CD32a)/免疫复合物/猪主动脉内皮细胞/凝血并发症/供者器官短缺Key words
Xenotransplantation/Coagulation dysfunction/Platelet/FcγRⅡa(CD32a)/Immune complex/Porcine aortic endothelial cell/Coagulation complication/Shortage of donor organ分类
医药卫生引用本文复制引用
赵利强,王权成,缑矗衡,张虹,洪昕,张玄,窦科峰..免疫复合物-血小板FcγRⅡa(CD32a)介导的异种移植相关凝血功能障碍机制的初步研究[J].器官移植,2026,17(3):405-412,8.基金项目
国家自然科学基金(82302005、82588101、82371793) （82302005、82588101、82371793）
四大慢病国家科技重大专项(2025ZD0552103) （2025ZD0552103）
国家重点研发计划项目(2024YFC3406802) （2024YFC3406802）
陕西省卫生健康委重点研发计划项目(2025YF-01) （2025YF-01）
第四军医大学人才助推项目(2023RCJB10) （2023RCJB10）
西京医院创新医学研究项目(XJZT24CY13) （XJZT24CY13）
