日用化学工业（中英文）2026，Vol.56Issue(4)：471-478,8.DOI:10.3969/j.issn.2097-2806.2026.04.007
黑色素生成抑制肽的设计筛选及功效评价研究
Design,screening and efficacy evaluation of melanin-production inhibitory peptides
董雨 1荣绍丰 1管世敏 1李茜茜1
作者信息
- 1. 上海应用技术大学香料香精化妆品学部,上海 201418
- 折叠
摘要
Abstract
Currently,there is little research on tetrapeptides with melanin inhibitory activity.This article aims to study melanin inhibition by constructing a virtual peptide library and establishing a design and screening method to select and evaluate tetrapeptides with melanin inhibitory activity.Based on a virtual peptide library,technologies such as molecular docking,activity prediction,and force analysis were used to screen the target active tetrapeptides,and then solid-phase peptide synthesis was employed for directed synthesis of the target tetrapeptides.The efficacy of the target active tetrapeptides was further evaluated from biochemical detection,cell models,and molecular transcription perspectives.Four tetrapeptides are selected:Peptide-1(CHWH),Peptide-2(RVGW),Peptide-3(LHSY),and Peptide-4(LFSY).Compared to the other three tetrapeptides,Peptide-1 exhibits better Cu2+chelation,ABTS positive ion scavenging,and tyrosinase inhibitory activity.The IC50 values for tyrosinase activity and melanin production inhibition in B16 cell models are 0.28 and 0.20 g/L,respectively,and it dose not significantly affect cell proliferation below a mass concentration of 10 g/L.Transcriptional studies also further show that Peptide-1 can decrease the intracellular levels of MITF(microphthalmia-associated transcription factor)and TYR(tyrosinase)gene expression in a concentration ration-dependent manner.关键词
活性四肽/B16细胞/酪氨酸酶活性抑制/黑色素生成Key words
active tetrapeptide/B16 cells/inhibition of tyrosinase activity/melanin production分类
化学化工引用本文复制引用
董雨,荣绍丰,管世敏,李茜茜..黑色素生成抑制肽的设计筛选及功效评价研究[J].日用化学工业（中英文）,2026,56(4):471-478,8.
