日用化学工业（中英文）2026，Vol.56Issue(4)：487-496,10.DOI:10.3969/j.issn.2097-2806.2026.04.009
一种中药生发液的毛囊刺激性及防脱生发功效探究
Exploration of hair follicle irritation and hair growth efficacy of a Traditional Chinese Medicine hair growth solution
摘要
Abstract
This study observed the effects of the same Traditional Chinese Medicine combination on hair papilla cells,keratinocytes,and isolated hair follicles under 7 different extraction methods,and explored which extraction method promotes the effect of promoting hair growth while causing less toxic damage to cells.The MTT assay was used to detect the toxicity and proliferative effects of the seven extraction solutions on dermal papilla cells and keratinocytes.Three extraction solutions with better cell viability enhancement effects at the 72-hour time point in the proliferation experiment were selected for hair follicle culture in vitro,and the growth of hair follicles and changes in hair follicle growth cycle were observed.Finally,the fingerprint spectrum of the Traditional Chinese Medicine formula was constructed using liquid chromatography-mass spectrometry,and the main chemical components were identified.The results show that the extraction method for 7 medicinal herbs with alcohol precipitation,and 3 medicinal herbs with alcohol extraction combined with 15 μL of volatile oil(G extraction solution)has a significant promoting effect on the growth of hair follicles in vitro.It also has a regulatory effect on the growth cycle of hair follicles by prolonging the growth period and slowing the degeneration period,thereby achieving hair growth.关键词
脱发/中药组合物/中药提取/离体毛囊Key words
hair loss/Traditional Chinese Medicine combination/Traditional Chinese Medicine extraction/in vitro hair follicle分类
化学化工引用本文复制引用
邹枫,张荣,陆颖珊,吴汶丰,梁家芬,李红毅..一种中药生发液的毛囊刺激性及防脱生发功效探究[J].日用化学工业（中英文）,2026,56(4):487-496,10.基金项目
国家中医药管理局中医学术流派传承工作室建设项目:岭南皮肤病流派传承工作室项目(LP0119045) （LP0119045）
