四川精神卫生2026，Vol.39Issue(2)：140-148,157,10.DOI:10.11886/scjsws20251114002
同时测定人血清中12种抗精神病药物及其主要代谢物的方法
A method for the simultaneous determination of 12 antipsychotic drugs and their main metabolites in human serum
摘要
Abstract
Background Monitoring the blood concentrations of antipsychotic drugs and their metabolites can guide the adjustment of clinical treatment plans,improving therapeutic efficacy while reducing adverse effects.However,there is currently a lack of a method that can accurately and efficiently quantitatively detect multiple antipsychotic drugs and their metabolites.Objective To establish a ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous identification and quantitation of 12 antipsychotic drugs and their main metabolites in human serum.Methods Using UPLC-MS/MS technology,protein precipitation method was employed for sample pretreatment.An Agela Technologies Durashell C8 chromatographic column(50 mm×3.0 mm,5 μm)was selected for chromatographic separation with gradient elution.The flow rate was 0.4 mL/min,and the total analysis time was 5 minutes.The column temperature was 40℃.The mass spectrometry detection was carried out in the multiple reaction monitoring(MRM)mode,and the isotope internal standard method was used for quantification.Results The relative standard deviation(RSD)of the internal standard normalization matrix effect factor for 12 antipsychotic drugs and their main metabolites at low and high quality concentrations was all less than 15%.The extraction recovery rate was 85%to 115%.They showed good linear relationships within their respective standard curve ranges(r>0.995).At low,medium,and high quality concentrations,the accuracy was 85.24%to 114.71%,and the RSD of intra-batch and inter-batch precision was all≤14.15%,with good stability.Conclusion All the analytical performance indicators of this method meet the verification requirements,providing an analytical means for the quantitative detection of antipsychotic drugs and their main metabolites in human serum.关键词
抗精神病药物/血清药物浓度/超高效液相色谱-串联质谱Key words
Antipsychotic drugs/Serum drug concentration/UPLC-MS/MS分类
医药卫生引用本文复制引用
陈曦,肖艳芳,丁仰,洪伟涛,麦丽君,曾璇..同时测定人血清中12种抗精神病药物及其主要代谢物的方法[J].四川精神卫生,2026,39(2):140-148,157,10.基金项目
2023年潮州市第三批科技专项(项目名称:基于药物基因组学和血药浓度监测指导的棕榈酸帕利哌酮长效针剂个体化用药研究,项目编号:202303GY02) Funded by The Third Batch of Science and Technology Projects in Chaozhou City in 2023(number,202303GY02) （项目名称:基于药物基因组学和血药浓度监测指导的棕榈酸帕利哌酮长效针剂个体化用药研究,项目编号:202303GY02）
