水产科技情报2026，Vol.53Issue(3)：147-154,8.DOI:10.16446/j.fsti.20250100121
大黄鱼内脏白点病病原分离鉴定及致病性研究
Isolation,identification and pathogenicity analysis of white spot disease in viscera of Larimichthys crocea
摘要
Abstract
In order to investigate the pathogenic bacteria and pathogenicity of visceral white spot disease of large yellow croaker(Larimichthys crocea),a dominant strain,DHS-FD-01,was isolated and purified from diseased large yellow croaker cultured in Fuding,Fujian Province.Physiological and biochemical analysis indicated that the strain was Pseudomonas.The strain was identified as P.plecoglossicida based on the sequencing results of 16S rRNA gene,gyrB,oprF,oprI,oprL and rpoD genes and phylogenetic analysis.Moreover,the strain could induce the occurrence of visceral white spot disease in L.crocea in the artificial infection test at water temperature of 15-20℃.DHS-FD-01 was highly sensitive to neomycin and ceftazidime.Obvious inflammatory and necrotic symp-toms were observed in the liver,spleen,head kidney of the diseased L.crocea.The results provide an experimen-tal basis for the scientific prevention and control of visceral white spot disease in L.crocea caused by this strain.关键词
大黄鱼/内脏白点病/杀香鱼假单胞菌/致病性/组织病理Key words
Larimichthy crocea/visceral white spot disease/Pseudomonas plecoglossicida/pathogenicity/histo-pathology引用本文复制引用
韩旭磊,宋炜,谢国驷,徐豪,王启要,吉群,王卓,李莉珍,崔相镐,黄少聪..大黄鱼内脏白点病病原分离鉴定及致病性研究[J].水产科技情报,2026,53(3):147-154,8.基金项目
国家重点研发计划(2023YFC2812108 （）
2022YFD2401102) （）
青岛海洋科技中心山东省专项经费(2022QNLM30001) （2022QNLM30001）
国家重点研发计划政府间国际科技创新合作重点专项(2022YFE0101200) （2022YFE0101200）
青岛海洋科技中心山东省专项经费(2022QNLM30001) （2022QNLM30001）
中国水产科学研究院基本科研业务费(2022TD76). （2022TD76）
