山东农业科学2026，Vol.58Issue(4)：46-53,8.DOI:10.14083/j.issn.1001-4942.2026.04.007
章丘大葱地方品种'大梧桐'染色体多样性分析
Chromosome Diversity Analysis of Zhangqiu Green Onion Landrace Dawutong
摘要
Abstract
Chromosome variation is one of the important genetic factors for crop evolution and breeding selection.The landrace Dawutong is a core germplasm of the national geographical indication cultivar Zhangqiu green onion(Allium fistulosum L.)with significant economic value.However,its chromosome constitution and diversity remain unclear.In this study,its chromosome characteristics were systematically analyzed using ge-nome sequencing and fluorescence in situ hybridization(FISH).The results were as follows.A total of 288 tandem repeat sequence clusters were identified and 15 oligonucleotide probes were designed.According to the signal patterns of these probes,an oligonucleotide probe multiplex containing nine probes were developed for distinguishing chromosomes of Dawutong,and a reference karyotype was constructed.Karyotyping analysis of 33 individual plants revealed that Dawutong had a high degree of karyotype variation among different individu-als.Chr1 to Chr4 each showed one karyotype,while the other four chromosomes contained 14 karyotypes.Chr8 had the most karyotypes(6),followed by Chr7(4),and Chr5 and Chr6 each contained 2 karyotypes.Three polymorphic types(Chr5-1,Chr6-1 and Chr8-1)had the frequency more than 50％,representing the domi-nant ones in Dawutong population.The average rates of chromosome homozygosity,heterozygosity and hetero-geneity were 67.51％,32.49％and 15.91％,respectively.Chr7 had the highest heterozygosity rate as 66.67％,and Chr8 had the highest heterogeneity rate as 27.27％,indicating high genetic diversity of this landrace.These results could provide an important technical support for Zhangqiu green onion germplasm identification,genetic research and improvement.关键词
章丘大葱/'大梧桐'/染色体荧光原位杂交/寡核苷酸探针/核型多样性Key words
Zhangqiu green onion/Dawutong/Fluorescence in situ hybridization/Oligonucleotide probe/Chromosome diversity分类
农业科技引用本文复制引用
张荣亭,张娟,任传猛,姚乐沙,卢红伟,张健刚,陈雪燕,李承永..章丘大葱地方品种'大梧桐'染色体多样性分析[J].山东农业科学,2026,58(4):46-53,8.基金项目
国家现代农业产业技术体系项目(CARS-24-12) （CARS-24-12）
济南市种业揭榜挂帅项目"济南市种质资源搜集、鉴定、保存和利用"(GG202403) （GG202403）
