山东医药2026，Vol.66Issue(4)：6-10,5.DOI:10.3969/j.issn.1002-266X.2026.04.002
子宫内膜癌组织中NORAD、FUBP1 mRNA表达变化及其意义
Expression changes of NORAD and FUBP1 mRNA in endometrial carcinoma tissues and their significance
摘要
Abstract
Objective To observe the expression changes of non-coding RNA activated by DNA damage(NORAD)and far-upstream element binding protein 1(FUBP1)mRNA in the endometrial carcinoma(EC)tissues,and to explore their clinical significance.Methods A total of 138 EC patients who underwent surgical treatment were enrolled.EC cancer tissues and adjacent non-cancerous tissues were collected during surgery.The qRT-PCR was used to detect the expression of NORAD,FUBP1 mRNA,as well as apoptosis-related genes Bax,p53,and Bcl-2 mRNA in EC cancer tissues and adjacent non-cancerous tissues.The correlation between the expression of NORAD and FUBP1 mRNA in EC cancer tissues,as well as their correlation with the expression of apoptosis-related genes Bax,p53,and Bcl-2 mRNA,was analyzed.Furthermore,the relationship between the expression of NORAD and FUBP1 mRNA and the clinicopathological parameters of EC patients was investigated.After discharge,patients were followed up until July 2025.The 3-year overall survival(OS)rate and 3-year progression-free survival(PFS)rate were calculated.The average relative expression levels of NORAD and FUBP1 mRNA were used as the cut-off value,the 138 EC patients were divided into the high and low NORAD expression groups,as well as the high and low FUBP1 mRNA expression groups.The relationships between the relative expression levels of NORAD and FUBP1 mRNA and the prognosis of EC patients were analyzed.Results The relative expression levels of NO-RAD and FUBP1 mRNA in EC cancer tissues were 0.82±0.20 and 3.11±0.63,respectively;the relative expression lev-els of NORAD and FUBP1 mRNA in adjacent non-cancerous tissues were 3.22±0.56 and 0.74±0.22,respectively;there were significant differences between these two kinds of tissues(all P<0.05).In EC cancer tissues,there was a negative correlation between the expression of NORAD and FUBP1 mRNA(r=-0.718,P<0.001).The relative expression levels of Bax and p53 mRNA in EC cancer tissues were lower than those in adjacent non-cancerous tissues,while the relative expres-sion level of Bcl-2 mRNA was higher(all P<0.05).In EC cancer tissues,there was a positive correlation between NORAD and the expression of Bax and p53 mRNA(r=0.662 and 0.651,respectively,both P<0.001),and a negative correlation with the expression of Bcl-2 mRNA(r=-0.712,P<0.001).In EC cancer tissues,FUBP1 mRNA expression was negative-ly correlated with the expression of Bax and p53 mRNA(r=-0.715 and-0.704,respectively,both P<0.001),and was positively correlated with the expression of Bcl-2 mRNA(r=0.656,P<0.001).The expression of NORAD and FUBP1 mRNA was associated with FIGO staging and lymph node metastasis in EC patients(both P<0.05).The 3-year PFS rate was higher in patients with high NORAD expression than in those with low NORAD expression(P<0.05),and the 3-year PFS rate was lower in patients with high FUBP1 mRNA expression than in those with low FUBP1 mRNA expression(P<0.05).Conclusions In EC cancer tissues,NORAD expression is down-regulated,FUBP1 mRNA expression is up-regula-ted,and there is a negative correlation between them.The expression of NORAD and FUBP1 mRNA in EC cancer tissues is correlated with the expression of apoptosis-related genes,as well as FIGO staging and lymph node metastasis in EC patients.EC patients with low NORAD expression and high FUBP1 mRNA expression have a poor prognosis.关键词
子宫内膜癌/DNA损伤激活的非编码RNA/远端上游元件结合蛋白1/凋亡/预后Key words
endometrial carcinoma/non-coding RNA activated by DNA damage/distal upstream element binding protein 1/apoptosis/prognosis分类
医药卫生引用本文复制引用
郭歌,李王丽,张慧,刘丽,白治苗..子宫内膜癌组织中NORAD、FUBP1 mRNA表达变化及其意义[J].山东医药,2026,66(4):6-10,5.基金项目
陕西省卫生健康科研项目(2020D0014). （2020D0014）
