山东医药2026，Vol.66Issue(4)：16-20,5.DOI:10.3969/j.issn.1002-266X.2026.04.004
三级淋巴结构评分在头颈部鳞状细胞癌预后评估及靶向治疗中的作用分析
Role of TLS score in prognosis evaluation and targeted therapy of head and neck squamous cell carcinoma
摘要
Abstract
Objective To investigate the immune characteristics of tertiary lymphoid structure(TLS)in head and neck squamous cell carcinoma(HNSCC),their prognostic value,and their associations with the tumor immune microenvi-ronment and mutational features,so as to provide a reference for the formulation of treatment strategies for HNSCC.Methods Transcriptomic data and clinical information of 522 tumor samples and 44 normal control samples were obtained from The Cancer Genome Atlas(TCGA)database.TLS scores were calculated using single-sample gene set enrichment a-nalysis(ssGSEA),and patients were stratified into the high-score TLS and low-score TLS based on the optimal cut-off val-ue.Survival analysis and Cox regression were performed to assess prognostic significance.Differentially expressed genes were subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.We evaluated the enrichment of immune cells using the Cell-type Identification by Estimating Relative Subsets of RNA Transcripts(CIBERSORT)algorithm and analyzed the status of the immune microenvironment under different TLS scores.We calculat-ed the tumor mutation burden(TMB)with maftools and analyzed its correlation with TLS scores.Meanwhile,the expression differences of immune checkpoint genes were compared.Results Overall survival was higher in the high-score TLS than in the low-score TLS(P<0.05).TLS score,age,and stage were independent influencing factors for clinical prognosis of HNSCC patients(all P<0.05).Differentially expressed genes were mainly enriched in tumor immunity-related pathways such as cyto-kine-cytokine receptor interaction,T-cell receptor signaling pathway,and B-cell receptor signaling pathway.The high-score TLS showed higher infiltration levels of multiple antitumor immune cells(all P<0.05).TLS score was negatively correlated with TMB(P<0.05).TP53,TTN,FAT1,and CDKN2A had high mutation frequencies.In addition,the expression levels of 11 immune checkpoint genes in the high-score TLS group were higher than those in the lows-core TLS group(all P<0.05).Conclusions TLS is closely associated with prognosis and the tumor immune microenvironment in HNSCC.TLS score may serve as a potential prognostic biomarker and provide insights for immunotherapy and targeted therapy.关键词
三级淋巴结构/头颈部鳞状细胞癌/生物标志物/免疫浸润/预后/靶向治疗Key words
tertiary lymphoid structure/head and neck squamous cell carcinoma/biomarkers/immune infiltration/prognosis/targeted therapy分类
医药卫生引用本文复制引用
王浩然,张梦琪,张宁,张东升,周晓清,徐乐..三级淋巴结构评分在头颈部鳞状细胞癌预后评估及靶向治疗中的作用分析[J].山东医药,2026,66(4):16-20,5.基金项目
山东省自然科学基金青年项目(ZR2022QH395). （ZR2022QH395）
