世界中医药2026，Vol.21Issue(3)：368-381,14.DOI:10.3969/j.issn.1673-7202.2026.03.002
不同制备工艺苓桂术甘汤化学成分分析与方剂配伍合理性分析
Chemical Composition of Linggui Zhugan Decoction with Different Preparation Processes and Rationality of Its Formula Compatibility
摘要
Abstract
Objective:To compare the similarities and differences in the material basis of Linggui Zhugan Decoction(LGZG)pre-pared using different methods,and to clarify the potential relationship between material distribution and compatibility based on net-work pharmacology.Methods:A targeted determination method for LGZG was established using ultra-high-performance liquid chro-matography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-QTOF-MS/MS).Qualitative and relative quantitative analyses were conducted on traditional benchmark samples and intelligent decoction samples.Key active ingredients were screened,and Gene Ontology(GO)enrichment analysis of targets related to non-alcoholic fatty liver disease(NAFLD)was performed using network pharmacology platforms,including SWISS.Results:Both the traditional benchmark and intelligent decoc-tion samples contained 142 components,with 91.89％overlap,indicating highly similar chemical profiles.Relative quantitative a-nalysis showed that the intelligent decoction process improved the dissolution of Poria cocos components,which were poorly soluble in the traditional water decoction.GO enrichment analysis revealed that P.cocos was associated with the largest number of genes and exhibited the highest betweenness centrality,suggesting it plays the principal therapeutic role,while the other herbs provide synergistic and auxiliary effects.Conclusion:Using UHPLC-QTOF-MS/MS,this study systematically analyzed the similarities and differences in the material basis of LGZG prepared by different methods at both qualitative and relative quantitative levels.Network pharmacology analysis clarified the rationale for LGZG compatibility in treating NAFLD from the perspective of material distribu-tion,providing a reference for chemical composition studies of other traditional Chinese medicine decoctions.关键词
经典名方/苓桂术甘汤/智能代煎/高效液相色谱-四极杆-飞行时间串联质谱法/全成分分析/网络药理学/方剂配伍/非酒精性脂肪肝Key words
Classic prescription/Linggui Zhugan Decoction/Intelligent decoction process/UHPLC-QTOF-MS/MS/Full compo-nent analysis/Network pharmacology/Formula compatibility/Non-alcoholic fatty liver disease分类
医药卫生引用本文复制引用
林学玉,许陈思菡,凌菲菲,杨凤林,李玲,张彤..不同制备工艺苓桂术甘汤化学成分分析与方剂配伍合理性分析[J].世界中医药,2026,21(3):368-381,14.基金项目
国家重点研发计划项目(2022YFC3501705) （2022YFC3501705）
国家自然科学基金面上项目(82274066) （82274066）
上海市领军人才计划项目(SHLJ2019100) （SHLJ2019100）
上海中医药大学第十七批大学生创新创业活动计划项目(2023SHUTCM100). （2023SHUTCM100）
