世界中医药2026，Vol.21Issue(3)：398-402,5.DOI:10.3969/j.issn.1673-7202.2026.03.005
桂枝茯苓汤对精索静脉曲张大鼠生精细胞凋亡的作用机制
Mechanisms of Guizhi Fuling Decoction on Spermatogenic Cell Apoptosis in Rats with Varicocele
摘要
Abstract
Objective:To investigate the effects of Guizhi Fuling Decoction on spermatogenic cell apoptosis in a rat model of vari-cocele(VC)via regulation of the vascular endothelial growth factor/phosphatidylinositol 3-kinase/protein kinase B(VEGF/PI3K/AKT)signaling pathway.Methods:Sprague Dawley(SD)rats were randomly divided into five groups:normal control group(NC),VC model group(VC),VC+Guizhi Fuling Decoction low-dose group(GZFL-L),VC+Guizhi Fuling Decoction medium-dose group(GZFL-M),and VC+Guizhi Fuling Decoction high-dose group(GZFL-H).Testicular testosterone levels were measured using an automatic biochemical analyzer.Apoptosis of spermatogenic cells in rat testes was detected using a TUNEL assay.The expression levels of VEGF,AKT,phosphorylated AKT(p-AKT),p70 ribosomal S6 kinase(p70S6K),and phosphorylated p70S6K(p-p70S6K)were determined by immunofluorescence or immunohistochemistry.Results:Compared with the NC group,testicular testosterone levels in the VC group were significantly decreased.After treatment with different doses of Guizhi Fuling Decoction,testosterone lev-els in the GZFL-L,GZFL-M,and GZFL-H groups were increased compared with those in the VC group,with the most significant in-crease observed in the GZFL-H group(P＜0.05).Compared with the NC group,the number of apoptotic spermatogenic cells was significantly increased in the VC group(P＜0.05).Compared with the VC group,the number of apoptotic cells in the GZFL-L,GZ-FL-M,and GZFL-H groups was significantly reduced(P＜0.05).Compared with the NC group,VEGF protein expression was in-creased in the VC group,whereas VEGF expression was significantly decreased in the GZFL-L,GZFL-M,and GZFL-H groups(P＜0.05).In addition,compared with the NC group,the expression levels of p-AKT and p-p70S6K were increased in the VC group(both P＜0.05).Compared with the VC group,the expression levels of p-AKT and p-p70S6K were decreased in the GZFL-L,GZ-FL-M,and GZFL-H groups(P＜0.05).Conclusion:Guizhi Fuling Decoction may inhibit spermatogenic cell apoptosis in VC rats by blocking the VEGF/PI3K/AKT signaling pathway.关键词
桂枝茯苓汤/血管内皮生长因子/磷脂酰肌醇3-激酶/蛋白激酶B信号通路/精索静脉曲张/生精细胞/凋亡/大鼠/睾丸/动物模型Key words
Guizhi Fuling Decoction/VEGF/PI3K/AKT pathway/Varicocele/Spermatogenic cells/Apoptosis/Rat/Testis/Ani-mal model分类
医药卫生引用本文复制引用
方茂霖,邢益涛,杨文涛..桂枝茯苓汤对精索静脉曲张大鼠生精细胞凋亡的作用机制[J].世界中医药,2026,21(3):398-402,5.基金项目
国家自然科学基金项目(81660796) （81660796）
海南省卫生计划行业科研项目(19A200083). （19A200083）
