世界中医药2026，Vol.21Issue(4)：590-596,606,8.DOI:10.3969/j.issn.1673-7202.2026.04.003
地黄饮子改善阿尔茨海默病肾虚精亏证大鼠神经炎症的作用机制
Mechanisms of Dihuang Yinzi Decoction in Improving Neuroinflammation in Rats with Alzheimer's Disease Characterized by Kidney Deficiency and Essence Depletion
摘要
Abstract
Objective:To investigate the mechanisms of Dihuang Yinzi Decoction on neuroinflammation in rats with Alzheimer's disease(AD)of kidney deficiency and essence depletion syndrome.Methods:Thirty-six rats were randomly divided into a control group,an AD of kidney deficiency and essence depletion syndrome model group,a low-dose Dihuang Yinzi Decoction group,a me-dium-dose Dihuang Yinzi Decoction group,a high-dose Dihuang Yinzi Decoction group,and a donepezil group using a random num-ber table.A combined disease-syndrome model was established by intraperitoneal injection of D-galactose to induce subacute aging in rats,together with bilateral hippocampal CA1 injection of β-amyloid fragment 25-35(Aβ25-3s)to establish an AD model with kid-ney deficiency and essence depletion syndrome.The Morris water maze was used to assess spatial learning and memory ability.Mi-croglial activation was detected by immunofluorescence.The expression levels of β-amyloid 1-42(Aβ1-42)and phosphorylated tau protein 404(pTau404)in the hippocampus were measured by immunohistochemistry.The levels of interleukin-1 β(IL-1 β)and tumor necrosis factor-α(TNF-α)in brain tissue were detected by enzyme-linked immunosorbent assay(ELISA).The protein ex-pression levels of AMP-activated protein kinase(AMPK),phosphorylated AMPK(p-AMPK),nuclear factor-κB p65(NF-κB p65),and phosphorylated NF-κB p65(p-p65)in the hippocampus were detected by Western blot.Results:Dihuang Yinzi Decoction sig-nificantly improved spatial learning and memory ability in AD rats with kidney deficiency and essence depletion syndrome.It im-proved hippocampal neuronal morphology and reduced mitochondrial swelling.The expression of ionized calcium-binding adaptor molecule l(Ibal)was decreased,indicating inhibition of microglial activation.The expression levels of Aβ1-42 and pTau404 were reduced.In addition,p-AMPK protein expression was increased,while p-p65 protein expression was decreased.Conclusion:Di-huang Yinzi Decoction may alleviate neuroinflammation in AD rats with kidney deficiency and essence depletion syndrome by acti-vating the AMPK/NF-κB signaling pathway,thereby exerting anti-AD effects.关键词
地黄饮子/阿尔茨海默病/肾虚精亏证/神经炎症/腺苷酸活化蛋白激酶/核因子κB/信号通路/β-淀粉样蛋白Key words
Dihuang Yinzi Decoction/Alzheimer's disease/Kidney deficiency and essence depletion syndrome/Neuroinflamma-tion/AMPK/NF-κB/Signaling pathway/β-amyloid分类
医药卫生引用本文复制引用
成金枝,高永强,贺文彬,闫金宙,陈伊旻,郭紫娟..地黄饮子改善阿尔茨海默病肾虚精亏证大鼠神经炎症的作用机制[J].世界中医药,2026,21(4):590-596,606,8.基金项目
国家自然科学基金青年科学基金项目(82004236) （82004236）
山西省基础研究计划自由探索类自然科学研究面上项目(202203021221286). （202203021221286）
