世界中医药2026，Vol.21Issue(4)：607-612,622,7.DOI:10.3969/j.issn.1673-7202.2026.04.005
补肾开窍祛痰方治疗缺氧缺血性脑损伤脑瘫的分子机制研究
Molecular Mechanisms of Bushen Kaiqiao Qutan Formula against Cerebral Palsy Induced by Hypoxic-ischemic Brain Damage
摘要
Abstract
Objective:To investigate the molecular mechanisms of Bushen Kaiqiao Qutan Formula(BSKQQTF)in treating a rat model of cerebral palsy induced by hypoxic-ischemic brain damage(HIBD).Methods:Rats were randomly divided into a normal group,a sham operation group,and a modeling group.The HIBD cerebral palsy model was established using the modified Rice-Van-nucci method.Successfully modeled rats were further randomly assigned to a model group,a Liuwei Dihuang Decoction group,and low-,medium-,and high-dose BSKQQTF groups.The drug intervention groups received intragastric administration starting on the second day after surgery,while the normal,sham operation,and model groups were administered an equal volume of normal saline for three consecutive weeks.Neurological function scores were evaluated at 1,2,and 3 weeks after modeling.The expression levels of Cytochrome C(Cyt-C),Caspase-9,Caspase-3,and apoptotic protease activating factor-1(Apaf-1)in the hippocampal CA1 region were assessed in each group.Results:Zea-Longa scores:Compared with the model group,the normal,sham operation,BSKQQTF(low-,medium-,and high-dose),and Liuwei Dihuang Decoction groups showed significantly decreased Zea-Longa scores at weeks 1 and 2(P＜0.05).Immunohistochemistry:Compared with the model group,positive expression of Cyt-C,Caspase-9,Caspase-3,and Apaf-1 proteins in the hippocampal CA1 region was reduced in the normal,sham operation,and medium-dose BSKQQTF groups.Western blot for hippocampal CAI region:Compared with the model group,protein expression levels of Cyt-C,Caspase-9,Caspase-3,and Apaf-1 were decreased in the medium-dose BSKQQTF group(P＜0.01).In the high-dose BSKQQTF and Liuwei Dihuang Decoction groups,Apaf-1 protein expression was also decreased(P＜0.01).Real-time quantitative PCR(qRT-PCR):Compared with the model group,mRNA expression levels of Cyt-C,Caspase-9,Caspase-3,and Apaf-1 in the hippocampal CA1 region were significantly reduced in the medium-dose BSKQQTF group(P＜0.01).Conclusion:BSKQQTF can treat HIBD-induced cerebral palsy,possibly by inhibiting neuronal apoptosis through suppression of activation of the Cyt-c/Caspase signaling pathway.关键词
脑性瘫痪/补肾开窍祛痰方/缺氧缺血性脑损伤/细胞色素C/胱天蛋白酶-9/胱天蛋白酶-3/凋亡蛋白酶活化因子-1/细胞凋亡Key words
Cerebral palsy/Bushen Kaiqiao Qutan Formula/Hypoxic-ischemic brain damage/Cyt-c/Caspase-9/Caspase-3/Apaf-1/Apoptosis分类
医药卫生引用本文复制引用
单海军,介小素,张英英,曹彩红,米晓阳,李宗泽,江凯静..补肾开窍祛痰方治疗缺氧缺血性脑损伤脑瘫的分子机制研究[J].世界中医药,2026,21(4):607-612,622,7.基金项目
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
河南省中医药科学研究专项课题(重点课题)(2019ZY1020). （重点课题）
