世界中医药2026，Vol.21Issue(4)：613-622,10.DOI:10.3969/j.issn.1673-7202.2026.04.006
姜黄素增强低糖低氧小胶质细胞M2极化的机制研究
Mechanisms of Curcumin in Enhancing M2 Polarization of Microglia under Low-glucose and Hypoxic Culture Conditions
摘要
Abstract
Objective:To preliminarily investigate the regulatory effects and possible mechanisms of curcumin on M2 polarization of microglia and inflammatory mediator expression under low-glucose and hypoxic culture conditions.Methods:A low-glucose and hy-poxia microglial culture model was established,and the effects of curcumin on inflammatory mediator expression in microglia under different durations of low-glucose and hypoxic culture were examined.A Toll-like receptor 4(TLR4)gene interference plasmid was constructed and transfected into microglia.After 18 hours of low-glucose and hypoxic culture,the protein expression of M1 and M2 markers,inflammatory mediators,and transcription factors was detected.Results:Under low-glucose and hypoxic culture conditions,curcumin promoted the expression of interleukin-4(IL-4)and inhibited the expression of inflammatory mediators tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β),and interleukin-6(IL-6).It also significantly suppressed TLR4 expression.TLR4 gene silencing further promoted curcumin-induced M2 polarization of microglia under low-glucose and hypoxia,and further enhanced the promoting effect of curcumin on IL-4 expression as well as its inhibitory effect on IL-1 β expression.In addition,curcumin signifi-cantly inhibited the protein expression of the detected transcription factors,and TLR4 gene silencing further enhanced its inhibitory effect on these transcription factors.Conclusion:Under low-glucose and hypoxic culture conditions,curcumin significantly promotes M2 polarization of microglia,enhances the expression of the anti-inflammatory factor IL-4,and inhibits the expression of the inflam-matory mediator IL-1β.关键词
慢性脑血流低灌注/姜黄素/小胶质细胞/白细胞介素-4/Toll样受体4/炎症介质Key words
Chronic cerebral hypoperfusion/Curcumin/Microglia/Interleukin-4(IL-4)/Toll-like receptor 4(TLR4)/Inflamma-tory factors分类
医药卫生引用本文复制引用
余美玲,李德跃,段炜..姜黄素增强低糖低氧小胶质细胞M2极化的机制研究[J].世界中医药,2026,21(4):613-622,10.基金项目
国家自然科学基金面上项目(81873757) （81873757）
重庆市自然科学基金项目(cstc2021jcyj-msxmX0494) （cstc2021jcyj-msxmX0494）
重庆市中青年高端医学人才项目(2022CQ016). （2022CQ016）
