世界中医药2026，Vol.21Issue(4)：623-628,635,7.DOI:10.3969/j.issn.1673-7202.2026.04.007
降酶退黄合剂治疗急性肝损伤的作用机制研究
Mechanisms of Jiangmei Tuihuang Mixture in Treating Acute Liver Injury
摘要
Abstract
Objective:To investigate the protective effects and mechanisms of Jiangmei Tuihuang Mixture(JTM)on hepatocytes in an acute liver injury model based on the miRNA-103/phosphatase and tensin homolog(PTEN)/endoplasmic reticulum stress(ERS)signaling pathway.Methods:Human hepatocyte cell line HHL-5 was routinely cultured and divided into a blank group,a model group,a JTM group,a miR-103-3p inhibitor negative control(NC)group,a miR-103-3p inhibitor group,and a miR-103-3p inhibitor+JTM group.JTM-containing serum was prepared.The CCK-8 assay was used to determine the optimal drug concentration and exposure time for intervention.Western blot(WB)and quantitative reverse transcription-polymerase chain reaction(qRT-PCR)were used to detect the mRNA and protein expression levels of miR-103-3p and PTEN/ERS pathway-related molecules in HHL-5 cells.Results:Compared with the blank group,the model group showed significantly increased mRNA and protein expression levels of miR-103-3p and PTEN/ERS pathway-related molecules(P＜0.05).Compared with the model group,the JTM group and the miR-103-3p inhibitor+JTM group showed decreased mRNA expression levels of miR-103-3p,PTEN,glucose-regulated protein 78(GRP78),protein kinase R-like endoplasmic reticulum kinase(PERK),activating transcription factor 4(ATF4),C/EBP homolo-gous protein(CHOP),activating transcription factor 6(ATF6),phosphorylated inositol-requiring enzyme 1(p-IREl),and X-box binding protein 1(XBP-1),with the most significant effect observed in the miR-103-3p inhibitor+JTM group(P＜0.01).Protein expression levels of PTEN,GRP78,PERK,ATF4,CHOP,ATF6,p-IREl,and XBP-1 were also decreased,with the miR-103-3p in-hibitor+JTM group showing a more significant effect(P＜0.05).Conclusion:JTM can exert hepatoprotective effects by downregu-lating miR-103-3p expression in hepatocytes with acute liver injury,thereby blocking PTEN/ERS signal transduction,preventing endoplasmic reticulum dysfunction,and ultimately protecting liver function.关键词
降酶退黄合剂/急性肝损伤/微小核糖核酸-103/磷酸酶及张力蛋白磷酸酶同源物/内质网应激/信号通路/内质网/细胞凋亡Key words
Jiangmei Tuihuang Mixture/Acute liver injury/MiRNA103/PTEN/ERS/Signaling pathway/Endoplasmic reticu-lum/Apoptosis分类
医药卫生引用本文复制引用
程玲,张琦,李魏,魏连波,程怀东..降酶退黄合剂治疗急性肝损伤的作用机制研究[J].世界中医药,2026,21(4):623-628,635,7.基金项目
国家自然科学基金项目(82274251) （82274251）
安徽省卫生健康科研项目(AHWJ2023A20149) （AHWJ2023A20149）
安徽省新时代育人质量工程(研究生教育)项目(2024zyxwjxalk116) （研究生教育）
深圳市科技计划项目(JCYJ20220530154203007) （JCYJ20220530154203007）
南方医科大学2024年腾飞计划项目(23H3ATF01) （23H3ATF01）
安徽中医药大学临床科研项目(2021yfylc04). （2021yfylc04）
