世界中医药2026，Vol.21Issue(4)：644-651,8.DOI:10.3969/j.issn.1673-7202.2026.04.010
壮医祛毒通养方对多囊卵巢综合征大鼠胰岛素抵抗的作用机制
Mechanisms of Qudu Tongyang Formula of Zhuang Medicine in Improving Insulin Resistance in Polycystic Ovary Syndrome Rats
摘要
Abstract
Objective:To investigate the mechanisms of the Zhuang medicine Qudu Tongyang Formula in rats with polycystic ovary syndrome and insulin resistance(PCOS-IR).Methods:Sixty female Sprague Dawley(SD)rats were randomly divided into a blank control group,a model control group,a positive control group,and low-,medium-,and high-dose Qudu Tongyang Formula groups,with 10 rats in each group.Except for the blank control group,all other groups were subjected to a high-fat diet combined with letro-zole gavage to establish the PCOS-IR model.After 21 days of intervention,estrous cycle changes were observed.Fasting blood glu-cose(FBG),fasting insulin(FINS),luteinizing hormone(LH),follicle-stimulating hormone(FSH),testosterone(T),and estradiol(E2)levels were measured,and the insulin resistance index(HOMA-IR)was calculated.The expression levels of phosphatidyli-nositol 3-kinase(PI3K)/protein kinase B(AKT)/glucose transporter 4(GLUT4)signaling pathway-related molecules in ovarian tissue were detected.Ovarian organ coefficients and pathological morphology were evaluated.Results:Compared with the blank con-trol group,the model control group showed estrous cycle disorder,polycystic ovarian changes,and reduced granulosa cell layers.FBG,FINS,HOMA-IR,LH,T levels,and ovarian coefficients were significantly increased(P＜0.05),while E2 and FSH levels and the mRNA expression of PI3K,AKT,GLUT4,as well as the protein expression of PI3K,AKT,p-AKT,and GLUT4,were significant-ly decreased(P＜0.05).Compared with the model control group,all dose groups of the Qudu Tongyang Formula showed improve-ment in ovarian pathology,with reduced cystic follicles and thickening of the granulosa cell layer.FBG,FINS,HOMA-IR,LH,T lev-els,and ovarian coefficients were significantly decreased(P＜0.05),while E2,FSH levels,and the above pathway-related mRNA and protein expression levels were significantly increased(P＜0.05).Conclusion:The Zhuang medicine Qudu Tongyang Formula can improve insulin resistance and alleviate polycystic ovarian changes in PCOS rats.Its mechanism may be related to activation of the PI3K/AKT/GLUT4 signaling pathway.关键词
壮医/壮医祛毒通养方/多囊卵巢综合征/磷脂酰肌醇3-激酶/蛋白激酶B/葡萄糖转运体信号通路/胰岛素抵抗Key words
Zhuang medicine/Qudu Tongyang Formula of Zhuang medicine/Polycystic ovary syndrome/PI3K/AKT/GLUT4/In-sulin resistance分类
医药卫生引用本文复制引用
范小婷,方刚,骆裕欣妍,邓肖彬,刘木青,韦丽丽,李清清,沈小淞..壮医祛毒通养方对多囊卵巢综合征大鼠胰岛素抵抗的作用机制[J].世界中医药,2026,21(4):644-651,8.基金项目
国家中医药管理局高水平中医药重点学科建设[少数民族医学(壮医学)]项目(zyyzdxk-2023164) （壮医学）
广西中医药大学2022年博士科研启动基金项目(2022BS014) （2022BS014）
广西高校壮医毒病研究重点实验室(桂教科研[2022]10号). （桂教科研[2022]10号）
