饲料研究2026，Vol.49Issue(7)：31-36,6.DOI:10.13557/j.cnki.issn1002-2813.2026.07.006
菌酶复合制剂对蛋鸡产蛋性能、营养物质表观代谢率、免疫功能及肠道健康的影响
Effects of a complex probiotic-enzyme preparation on laying performance,nutrient apparent metabolic rate,immune function,and intestinal health of laying hens
摘要
Abstract
The experiment aimed to investigate the effects of a complex probiotic-enzyme preparation composed of Bacillus licheniformis,Bacillus subtilis,and β-mannanase on the laying performance,nutrient apparent metabolic rate,immune function,and intestinal health of laying hens.A total of 384 healthy 70-week-old Hy-Line brown laying hens with similar body condition were randomly divided into four groups,with six replicates per group and 16 hens per replicate.The control group(group CK)was fed a basal diet,while the low-dose(group L),medium-dose(group M),and high-dose groups(group H)were fed the basal diet supplemented with 0.02%complex probiotic agent+0.001%β-mannanase,0.04%complex probiotic agent+0.001%β-mannanase,and 0.08%complex probiotic agent+0.001%β-mannanase,respectively.The pre-test period was seven days,and the formal test period was 56 days.The results showed that compared with group CK,the feed-to-egg ratio in group M was significantly reduced(P<0.05),and the apparent metabolic rates of dry matter,crude protein,crude fat,and phosphorus were significantly increased(P<0.05).The levels of immunoglobulin A(IgA)and immunoglobulin M(IgM)in serum were significantly increased(P<0.05),and the activities of α-amylase and lipase in the duodenum were significantly increased(P<0.05).There were no significant differences in bacterial and fungal Alpha diversity in groups L,M,and H(P>0.05).At the fungal phylum level,compared with group CK,the relative abundance of Ascomycota in groups L,M,and H was increased,while the relative abundances of Basidiomycota and Chytridiomycota were decreased.At the fungal genus level,compared with group CK,the relative abundances of Leptobacillium,Fusarium,and Talaromyces in groups L and M were decreased,while the relative abundances of Pichia and Galactomyces were increased.The study shows that supplementation of the basal diet with 0.04%complex probiotic agent+0.001%β-mannanase can effectively improve laying performance,nutrient apparent metabolic rate,and immune function,modulate intestinal microbiota structure,and increase the relative abundance of beneficial bacteria of laying hens.关键词
蛋鸡/菌酶复合制剂/产蛋性能/营养物质表观代谢率/免疫功能/肠道菌群Key words
laying hens/complex probiotic-enzyme preparation/laying performance/nutrient apparent metabolic rate/immune function/intestinal microbiota分类
农业科技引用本文复制引用
林凯欣,崔佳田,程博,苏梓艺,付育,赵志敏,张春晓..菌酶复合制剂对蛋鸡产蛋性能、营养物质表观代谢率、免疫功能及肠道健康的影响[J].饲料研究,2026,49(7):31-36,6.基金项目
河北省农业农村厅农业科技成果转化资金项目(项目编号:2025JNZ-S26) （项目编号:2025JNZ-S26）
黑龙江省科学技术厅重点研发计划项目(项目编号:2024ZX02C22) （项目编号:2024ZX02C22）
