聚二甲基硅氧烷微流控适配体传感器:开启生物标志物即时检验新时代
Polydimethylsiloxane microfluidic aptasensor:opening a new era of biomarker point-of-care testing
摘要
Abstract
Microfluidic technology,an emerging micro-nano technology,facilitates the precise manipulation of trace liquids via diverse microstructures.It is distinguished by its miniaturization and cost-effectiveness.Among the synthetic materials employed for microfluidic chips,polydimethylsiloxane(PDMS),a polymer material,has emerged as the optimal choice for fabricating microfluidic chips.This preference stems from its straightforward manufacturing process,high transparency,outstanding chemical stability,and biocompatibility.Biosensors developed by in-tegrating aptamers,which serve as specific biological receptors,into PDMS microfluidic chips are termed PDMS microfluidic aptasensors.These aptasensors capitalize on the distinctive advantages of aptamers as biological recognition elements in conjunction with microfluidic technology.Mi-crofluidic technology transforms the interactions between biomolecules into readable signals that are readily processed and reported,thereby offering sensing methods characterized by high specificity and sensitivity.This advancement significantly propels the development of point-of-care testing(POCT)for biomarkers,enabling extensive applications in areas such as cancer screening and pathogen detection,and achieving rapid,accurate,and portable testing.The attributes of PDMS microfluidic aptasensors encompass low cost,minimal consumption,short processing time,and disposability.These features effectively curtail the consumption of samples and reagents and shorten the testing duration in the POCT realm.Moreover,optimizing microchannel designs,aptamer immobilization methods,and signal amplification strategies can further enhance the performance of PDMS microfluidic aptasensors and expand their detection range.This paper elaborately expounds on the definition and development of microfluidic technology,the application research of PDMS materials and their preparation processes in microfluidic chip manufacturing,as well as the de-velopment and processing of PDMS microfluidic aptasensors.It also enumerates the applications of optical,electrochemical,and dual-mode PDMS microfluidic aptasensors based on dual detection modes in the field of biomarker POCT,thus providing theoretical support for the future development and application of novel microfluidic aptasensors.关键词
微流控芯片/适配体传感器/聚二甲基硅氧烷/即时检验/生物标志物Key words
microfluidic chip/aptasensor/polydimethylsiloxane(PDMS)/point-of-care testing(POCT)/biomarker分类
化学化工引用本文复制引用
杨元超,肖人方,吴洋涛,白丽,王兴亚,翟景波..聚二甲基硅氧烷微流控适配体传感器:开启生物标志物即时检验新时代[J].色谱,2026,44(5):499-514,16.基金项目
内蒙古自治区重点研发和成果转化计划项目(2025YFSH0054) （2025YFSH0054）
2024中央支持地方高校科研平台项目——人兽共患病防控自治区高等学校重点实验室项目 （）
内蒙古自治区直属高校基本科研业务费项目——布病防治工程技术研究中心科研创新平台能力建设项目(NMDZK24002) （NMDZK24002）
新时代"科尔沁英才"工程创新团队项目(KEQYC20240202) （KEQYC20240202）
内蒙古自治区科技重大专项项目(2019ZD006) （2019ZD006）
内蒙古自治区研究生教育教学改革项目(JG2024045C). Key R&D and Achievement Transformation Plan Project of Inner Mongolia Autonomous Region(No.2025YFSH0054) （JG2024045C）
2024 Local Universities'Scientific Research Platform Project Supported by Central Government—Key Laboratory Project for Higher Universities of Inner Mongolia Autonomous Region （）
Basic Scientific Research Project for Universities of Inner Mongolia Autonomous Region—Capacity Building Project of Scientific Research and Innovation Platform of Brucellosis Prevention and Treatment Engineering Technology Research Center(No.NMDZK24002) （No.NMDZK24002）
New Era"Horqin Talent"Project Innova-tive Team Project(No.KEQYC20240202) （No.KEQYC20240202）
Major Science and Technology Projects of Inner Mongolia of China(No.2019ZD006) （No.2019ZD006）
Postgraduate Education and Teaching Reform Project of Inner Mongolia Au-tonomous Region(No.JG2024045C). （No.JG2024045C）
