色谱2026，Vol.44Issue(5)：515-524,10.DOI:10.3724/SP.J.1123.2025.04001
细胞表面糖蛋白相互作用规模化解析新技术及应用
Novel technologies for large-scale analysis of cell surface glycoprotein interactions and their applications
摘要
Abstract
Cell surface glycoprotein complexes regulate critical biological processes such as in-tercellular recognition,signal transduction,and immune modulation through dynamic glycosylation modifications.Their spatiotemporally specific in vivo interaction networks directly influence disease pathogenesis and therapeutic responses.However,studying these interactions faces substantial technical challenges due to the weak/transient nature of glycoprotein interactions and the complexity of glycosylation modifications.In this study,we employed membrane-impermeable crosslinkers combined with oxidized glycan-hydrazide specific reactions and in vivo chemical crosslinking-mass spectrometry technology,successfully achieving high-selectivity capture of glycoprotein interactions on living cell surfaces.This approach identified 4 457 high-confidence glycosylation sites corre-sponding to 1 637 glycoproteins in HeLa cells,with 84%localized to the cell surface.Further analysis revealed that over 90%of the identified protein-protein interaction networks comprised glycoprotein-glycoprotein interactions,glycoprotein-associated protein interactions,and associated protein interaction networks.Key glycoprotein-mediated interactions,such as those involving integrin beta-1(ITGB1),were elucidated.This methodology provides crucial insights for deciphering glycan functionality in cell surface glycoprotein interactions,contributes to revealing the pivotal role of glycosylation in biological processes like cellular signaling and immune regulation,and offers novel strategies for early disease diagnosis and precision therapeutics.关键词
原位化学交联/糖蛋白相互作用/选择性富集/液相色谱-串联质谱Key words
in vivo chemical crosslinking/glycoprotein interactions/selective enrichment/liquid chromatography-tandem mass spectrometry(LC-MS/MS)分类
化学化工引用本文复制引用
刘祎,吴慈,赵群,梁振,张付云,张丽华..细胞表面糖蛋白相互作用规模化解析新技术及应用[J].色谱,2026,44(5):515-524,10.基金项目
国家重点研发计划项目(2022YFC3401204) （2022YFC3401204）
医工联合创新基金(DMU-2&DICP UN202301). National Key Research and Development Program of China(No.2022YFC3401204) （DMU-2&DICP UN202301）
Medical-Engineering Joint Innovation Fund(No.DMU-2&DICP UN202301). （No.DMU-2&DICP UN202301）
