中国生态农业学报（中英文）2026，Vol.34Issue(5)：999-1012,14.DOI:10.12357/cjea.20250814
红豆草根际可培养细菌多样性及其植物促生抗旱功能
Diversity of culturable rhizosphere bacteria associated with Onobrychis viciifolia and their roles in plant growth promotion and drought tolerance
摘要
Abstract
Drought,one of the major abiotic stresses affecting plant growth and development,severely restricts crop productivity.This study aimed to screen plant growth-promoting rhizobacteria(PGPR)with excellent growth-promoting and drought-resistant traits from the rhizosphere soil of Onobrychis viciifolia(sainfoin)in different drought habitats,and to evaluate their regulatory effects on the growth,development,and physiological responses of Medicago sativa seedlings under drought stress.A total of 903 culturable bacterial strains belonging to 82 species were isolated via multi-medium cultivature and identified via 16S rRNA gene sequencing.These strains were predominantly affiliated to the phyla Proteobacteria,Actinobacteria,and Bacteroidetes,with the genera Serratia and Pseudomonas being dominant.Analysis of plant growth-promoting traits revealed that the 82 rhizobacterial strains exhibited varying degrees of plant growth-promoting properties.Specifically,strains OvR165(Enterobacter sp.),OvR548(Acinetobacter sp.)and OvR687(Pseudomonas sp.)possessed the capabilities of phosphate solubilization,nitrogen fixation,siderophore production,indole-3-acetic acid(IAA)secretion and extracellular polysaccharide(EPS)synthesis.Among them,OvR548 and OvR687 also exhibited strong biofilm formation abilities.Pot experiments demonstrated that inoculation with these three strains significantly alleviated the inhibitory effects of drought on Medicago sativa seedlings.The inoculation of the strains increased the plant height,root length,leaf area,biomass accumulation,chlorophyll content,and relative water content,while reducing the cell membrane damage and reactive oxygen species(ROS)accumulation,thus effectively mitigating drought stress-induced damage to plants.Additionally,these strains showed strong potential for improving plant tolerance to drought,further enhancing the ecological adaptability of crops under water scarcity conditions.In conclusion,the rhizosphere of O.viciifolia in different arid regions of Qinghai Province harbors abundant and functionally diverse resources of plant growth-promoting and drought-tolerant bacteria,which have great ecological value and application prospects.This study provides excellent strain resources and theoretical support for sustainable grassland management and microbial agent development in arid regions,helping to improve the productivity and resilience of crops in drought-prone areas.关键词
红豆草/植物促生根际细菌/分离鉴定/促生特性/紫花苜蓿Key words
Onobrychis viciifolia/plant growth-promoting rhizobacteria/isolation and identification/growth-promoting traits/Medicago sativa分类
农业科技引用本文复制引用
李阳,陈靓,师贞朋,李蛟,李文婧,庾海洋,李银鑫,殷恒霞..红豆草根际可培养细菌多样性及其植物促生抗旱功能[J].中国生态农业学报（中英文）,2026,34(5):999-1012,14.基金项目
国家自然科学基金项目(32300269)、青海省科技国际合作项目(2024-HZ-811)和青海大学科研实力提升专项(2026KTSA02)资助 This study was supported by the National Natural Science Foundation of China(32300269),the Specific Program for International Cooperation in Science and Technology,Qinghai Province(2024-HZ-811),and the Research Capacity Promotion Project of Qinghai University(2026KTSA02). （32300269）
