山西大学学报（自然科学版）2026，Vol.49Issue(3)：451-467,17.DOI:10.13451/j.sxu.ns.2026003
基于网络药理学探究参苓白术丸改善肌少症的关键活性成分
Exploring the Key Active Components of Shenling Baizhu Pill to Improve Sarcopenia Based on Network Pharmacology
摘要
Abstract
This study aims to explore the key active components of Shenling Baizhu Pill(SLBZP)in treating sarcopenia.The study utilizes Ultra-performance Liquid Chromatography/Quadrupole Time-of-flight Mass Spectrometry(UPLC-Q/TOF-MS)to analyze the chemical components in the extract of SLBZP;combines network pharmacology to predict the effective components and poten-tial core targets of SLBZP to improve sarcopenia;and employs a dexamethasone-induced zebrafish sarcopenia model to evaluate the sarcopenia-improving activity of SLBZP.Reverse Transcription-polymerase Chain Reaction(RT-PCR)is used to detect the expres-sion of related genes.The results show that 87 compounds were detected in the extract of SLBZP;network pharmacology analysis reveals that five potential active components in SLBZP,including genistein,diosgenin III,diosgenin IV,naringenin,and kaempferol,primarily act on targets such as AKT serine/threonine kinase 1(AKT,AKT1),interleukin-6(IL-6),F-Box Protein 32(FBXO32),and Tripartite Motif Containing 63(TRIM63).Molecular docking indicates that naringenin has a high binding affinity with FBXO32 pro-tein,and molecular dynamics simulations further reveal that the naringenin-FBXO32 complex exhibits good stability.Animal experi-ments on zebrafish demonstrate that naringenin significantly enhances the locomotor ability of the sarcopenia zebrafish model,im-proves muscle density in the model animals,and downregulates the mRNA expression of FBXO32 and TRIM63.In summary,this study is the first discovery to identify naringenin in Shenling Baizhu Pill as a potential key pharmacodynamic component for improv-ing sarcopenia,providing a theoretical basis for revealing the improve sarcopenia active components and mechanisms of action of SLBZP.关键词
参苓白术丸/肌少症/分子动力学/分子对接/柚皮素Key words
Shenling Baizhu Pill/sarcopenia/molecular dynamics/molecular docking/naringenin分类
医药卫生引用本文复制引用
金雨萌,韩利文,李晖,王传娜,周子融,李欣蕊,赵璠瑜,张泽阳,刘铠瑞,邓志鹏..基于网络药理学探究参苓白术丸改善肌少症的关键活性成分[J].山西大学学报（自然科学版）,2026,49(3):451-467,17.基金项目
国家中医药管理局科技司-山东省卫生健康委员会共建中医药科技项目(GZY-KJS-SD-2023-088) （GZY-KJS-SD-2023-088）
山东省自然科学基金(ZR2025MS1268) （ZR2025MS1268）
山东第一医科大学学术提升计划(2019LJ003) （2019LJ003）
