山西大学学报（自然科学版）2026，Vol.49Issue(3)：468-486,19.DOI:10.13451/j.sxu.ns.2026011
基于高分辨质谱代谢组学及网络药理学的蝉蜕质量评价
Quality Evaluation of Cicadae Periostracum Based on High-resolution Mass Spectrometry Metabolomics and Network Pharmacology
摘要
Abstract
Cicadae Periostracum,the exuviae shed by nymphs of Cryptotympana pustulata Fabricius during eclosion,has been used in traditional medicine for over a thousand years.Currently,its quality is evaluated primarily based on morphological characteristics,lacking standardized and scientifically robust quality control indicators linked to efficacy.In this study,authentic Cicadae Periostra-cum samples from eight major producing areas were collected and identified by morphological and microscopic examination.Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)was employed for metabolomic profiling,and quality assessment was performed by integrating metabolomics with network pharmacology.A total of 52 N-acetyldopamine compounds(from monomers to pentamers),29 linear dipeptides,and 6 amino acids were identified.Samples from Kaifeng(Henan),Guang'an(Sichuan),and Baoding(Hebei)exhibited relatively higher contents of N-acetyldopamine compounds,while samples from Changzhi(Shanxi)and Guang'an(Sichuan)showed significantly higher levels of characteristic peptides and amino acids.Further network pharmacology analysis revealed that N-acetyldopamine and dipeptide com-ponents act on ten core targets,including Sarcoma Proto-oncogene Tyrosine-Protein Kinase(SRC)and Phosphatidylinositol-3-Ki-nase Catalytic Subunit Alpha(PIK3CA),potentially exerting pharmacological effects through pathways such as neuroactive ligand-receptor interactions.Moreover,these components demonstrated stronger correlations with pharmacological activities.In conclusion,the quality of Cicadae Periostracum from the four regions—Kaifeng(Henan),Guang'an(Sichuan),Baoding(Hebei),and Changzhi(Shanxi)—appears superior,consistent with the traditional notion that"larger specimens are preferable for medicinal use."This study establishes an integrated strategy combining high-resolution mass spectrometry metabolomics and network pharmacology for quality evaluation of Cicadae Periostracum,providing new scientific evidence for its quality assessment.关键词
蝉蜕/N-乙酰多巴胺类/二肽/结构鉴定/质量波动Key words
cicadae periostracum/N-acetyldopamine analogs/dipeptide/structure identification/quality fluctuation分类
医药卫生引用本文复制引用
张琰,张玮玮,韩荣荣,刘春冶,李震宇..基于高分辨质谱代谢组学及网络药理学的蝉蜕质量评价[J].山西大学学报（自然科学版）,2026,49(3):468-486,19.基金项目
国家青年岐黄学者项目(2022-256) （2022-256）
山西省中医药管理局课题(zyytd2024020) （zyytd2024020）
山西药科职业学院院级课题(课题编号2025120) （课题编号2025120）
