山西医科大学学报2026，Vol.57Issue(4)：368-378,11.DOI:10.13753/j.issn.1007-6611.2026.04.002
基于网络药理学与数据挖掘的半夏泻心汤抗胃癌关键靶点及作用机制
Key target genes and role mechanism of Banxia Xiexin decoction against gastric cancer based on network pharmacology and data mining
摘要
Abstract
Objective To explore core target genes and potential mechanisms of Banxia Xiexin decoction against gastric cancer(GC)based on network pharmacology and data mining.Methods Potential targets of Banxia Xiexin decoction against gastric cancer were screened based on network pharmacology and The Cancer Genome Atlas(TCGA)database analysis.Key target genes were then screened based on betweenness centrality(BC),degree centrality(DC),and closeness centrality(CC)scores.Subsequently,Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed.The top three most significant genes(P<0.05)identified by survival analysis were selected as core targets,and their rela-tionships with TNM stages and immune cell infiltration in GC were examined.Finally,the binding of the proteins encoded by the core targets to their corresponding ligands were validated through molecular docking.Results Of 1 115 potential targets of Banxia Xiexin decoction,171 associated with GC were identified,and the 13 key targets were further identified,including EZH2,FN1 and SER-PINE1.KEGG enrichment analysis indicated that these targets primarily participate in cancer-related pathways,as well as IL-17 and HIF-1 signaling pathways.Survival difference analysis demonstrated that EZH2,FN1 and SERPINE1 were core target genes,all of which were significantly correlated with T stage and immune cell infiltration in GC.Molecular docking studies confirmed that the pro-teins encoded by these three core targets exhibited strong binding affinities to their respective ligands.Conclusion EZH2,FN1,and SERPINE1 were identified as core targets of Banxia Xiexin decoction against gastric cancer via network pharmacology and data mining.The therapeutic mechanism may involve the regulation of cancer-related pathways,including IL-17 and HIF-1 signaling.Fur-thermore,the expression of these genes in gastric cancer correlates with T stage and immune infiltration,and their encoded proteins show strong binding to the decoction's active components.关键词
半夏泻心汤/胃癌/网络药理学/数据挖掘/作用机制/免疫细胞Key words
Banxia Xiexin decoction/gastric cancer/network pharmacology/data mining/mechanism of action/immune cells分类
医药卫生引用本文复制引用
涂泽宇,张淑敏,杨培培,黄逍,滕钰浩,徐媛媛,舒鹏..基于网络药理学与数据挖掘的半夏泻心汤抗胃癌关键靶点及作用机制[J].山西医科大学学报,2026,57(4):368-378,11.基金项目
癌症、心脑血管、呼吸和代谢性疾病防治研究国家科技重大专项(2024ZD0521300) （2024ZD0521300）
国家自然科学基金项目(82374539) （82374539）
江苏省中医药科技发展计划重点项目(ZD202214) （ZD202214）
江苏省中医药领军人才培养对象项目(SLJ0327) （SLJ0327）
南京中医药大学胃癌临床专病研究院项目(LCZBYJYZZ2024-001) （LCZBYJYZZ2024-001）
