山西医科大学学报2026，Vol.57Issue(4)：398-404,7.DOI:10.13753/j.issn.1007-6611.2026.04.005
异源脱细胞基质心肌补片改善大鼠心肌梗死后心功能
Xenogeneic decellularized matrix myocardial patch improves cardiac function in rats with myocardial infarction
摘要
Abstract
Objective To explore the therapeutic effect and underlying mechanism of a porcine-derived decellularized extracellular matrix(dECM)patch in rats with myocardial infarction(MI).Methods Porcine cardiac dECM patches were prepared using a physico-chemical combined method.SD rats were randomly divided into sham group,MI group,and MI+dECM group.Four weeks after surgery,cardiac function was assessed by echocardiography,including left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),and left ventricular internal dimension at end-systole(LVIDs).Pathological changes of myocardial tissue were observed by hematoxylin-eosin and Masson staining.The in vivo immune response was evaluated by immunofluorescence staining and the expres-sion of key proteins in the PI3K/Akt pathway was detected by Western blot.Results The rat models of myocardial infarction and dECM patch transplantation were successfully established.Compared with sham group,MI group had significantly decreased LVEF and LVFS(P<0.05),remarkably increased LVIDs(P<0.05),aggravated myocardial fibrosis,significantly increased myocardial infarct size(P<0.05),and notably down-regulated expression of p-PI3K and p-Akt proteins(P<0.05).Compared with MI group,LVEF and LVFS significantly increased while LVIDs significantly reduced in MI+dECM group(all P<0.05);the myocardial fibrosis was allevi-ated and myocardial infarct size reduced in MI+dECM group(P<0.05);no obvious immune cell infiltration was observed in MI+dECM group;moreover,the expression levels of p-PI3K and p-Akt were significantly up-regulated(P<0.05).Conclusion The xenogeneic dECM patch may improve cardiac function and reduce myocardial fibrosis after myocardial infarction by activating the PI3K/Akt signaling pathway.And it does not cause obvious immune rejection,showing good tissue compatibility.These findings suggest that the xenogeneic dECM patch is a promising myocardial repair material for clinical application.关键词
异源脱细胞基质补片/心肌梗死/PI3K/Akt信号通路/心功能/心脏修复Key words
xenogeneic dECM patch/myocardial infarction/PI3K/Akt signaling pathway/cardiac function/myocardial repair分类
医药卫生引用本文复制引用
张静,李倩,姜馨,聂建..异源脱细胞基质心肌补片改善大鼠心肌梗死后心功能[J].山西医科大学学报,2026,57(4):398-404,7.基金项目
博士科研启动经费资助项目(2024BS-16) （2024BS-16）
