山西医科大学学报2026，Vol.57Issue(4)：438-444,7.DOI:10.13753/j.issn.1007-6611.2026.04.009
川芎嗪对大鼠异位子宫内膜组织生长的抑制作用及其机制
Inhibitory effect and underlying mechanism of tetramethylpyrazine on ectopic endometrial tissue growth in rats
摘要
Abstract
Objective To investigate the inhibitory effect of tetramethylpyrazine(TMP)on the growth of ectopic endometrial tissue in rats with endometriosis,and to explore its underlying mechanism by examining the expressions of vascular endothelial growth factor A(VEGFA)and tumor necrosis factor-alpha(TNF-α).Methods An endometriosis model was established in Sprague-Dawley(SD)rats using autologous endometrial implantation.The successfully modeled rats were randomly divided into model group,low-,medium-,and high-dose TMP groups,with 6 rats in each group.The rats in TMP groups were administered with tetramethylpyrazine phosphate solution(1 mL/d)at concentrations of 2.375,4.75,9.5 mg/mL by gavage for 28 consecutive days,respectively,while the rats in model group received an equal volume of normal saline.After the intervention,the volume of ectopic endometrial tissue was mea-sured,and the volume reduction rate was calculated.Histopathological changes were observed using hematoxylin-eosin(HE)staining.The protein levels of VEGFA and TNF-α in ectopic endometrial tissue were detected by immunohis to chemistry,and those in serum and peritoneal lavage fluid were mearured by enzyme-linked immunosorbent assay(ELISA),respectively.Results Compared with model group,the volume reduction rate of ectopic endometrial tissue in medium-and high-dose TMP groups was significantly increased(P<0.05),and the volume reduction rate dose-dependently increased by TMP(P<0.05).Compared with model group,the expression levels of VEGFA and TNF-α in both ectopic endometrial tissue and serum significantly decreased in high-dose TMP group(P<0.05);how-ever,there was no statistically significant difference in the levels of VEGFA and TNF-α in peritoneal lavage fluid among all groups(P>0.05).Conclusion The medium-and high-dose TMP by gavage can effectively inhibit the growth of ectopic endometrial tissue in rats,accompanied by a decrease in the expression levels of VEGFA and TNF-α in both ectopic endometrial tissue and serum.Its mechanism of action may be associated with the inhibition of angiogenesis and the inflammatory microenvironment.关键词
川芎嗪/子宫内膜异位症/异位内膜组织/血管内皮生长因子A/肿瘤坏死因子-α/大鼠Key words
tetramethylpyrazine/endometriosis/ectopic endometrial tissue:vascular endothelial growth factor A(VEGFA)/tumor necrosis factor-alpha(TNF-α)/rats分类
医药卫生引用本文复制引用
房珊珊,曹建芳,高艳萍,李雅清,梁铭,陈琛..川芎嗪对大鼠异位子宫内膜组织生长的抑制作用及其机制[J].山西医科大学学报,2026,57(4):438-444,7.基金项目
大同市应用基础研究计划项目(2022058) （2022058）
大同市平台(基地)计划项目(2022085) （基地）
