时珍国医国药2026，Vol.37Issue(9)：1616-1623,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0903
逍遥散调控海马NLRP3炎症小体通路抗抑郁药效的机制研究
Antidepressant effects of Xiaoyao Powder(逍遥散)through the Hippocampal NLRP3 inflam-masome pathway
摘要
Abstract
Objective To investigate the antidepressant effects of Xiaoyao Powder(逍遥散,XYP),a classical traditional Chinese medicine(TCM)prescription,on chronic restraint stress(CRS)-induced depressive-like behaviors in mice and its regulatory effects on the NLRP3 inflammasome signaling pathway in the hippocampus.Methods Depressive-like behaviors in mice were evaluated using behavioral tests,including the open field test(OFT),tail suspension test(TST),forced swim test(FST),and sucrose preference test(SPT).The expression of key inflammatory markers in the hippocampus was assessed via quantitative PCR(qPCR)for Nlrp3,Il-18,Il-1β,Arg1,Cd206,Cd86,Inos,Pparg,and Ppargc1a,as well as Western blot(WB)for NLRP3,cleaved-Caspase-1/Caspase-1,IL-1β,ASC,and PPARγ.Results Mice in the CRS group exhibited pronounced depressive-like behaviors compared to the control group.Treatment with XYP or fluoxetine significantly ameliorated these behavioral abnormalities.qPCR analysis revealed that XYP markedly suppressed the hippocampal expression of the pro-inflammatory markers Nlrp3,Il-18,Il-1β,and M1 microglial markers Cd86 and Inos,while restoring the expression of M2 markers Arg1 and Cd206,as well as nuclear receptor Pparg and its coactivator Ppargc1a.Furthermore,XYP administration effectively inhibited the activation of the NLRP3/Caspase-1/ASC inflammasome pathway and the expression of IL-1β while enhancing PPARγ levels.Conclusion These findings suggest that XYP exerts antidepressant effects by modulating the Hippocampal NLRP3 inflammasome and associated signaling pathways.This study provides novel mechanistic insights and experimental evidence for supporting the clinical application of XYP in the treatment of depression.关键词
逍遥散/NLRP3炎症小体/抑郁症/慢性束缚应激/小胶质细胞Key words
Xiaoyao Powder(逍遥散)/NLRP3 inflammasome/Depression/Chronic restraint stress/Microglia分类
医药卫生引用本文复制引用
朱文俊,袁艿君,汤曼诗,唐自豪,佘楷杰,黄敏仪,马庆宇,陈家旭..逍遥散调控海马NLRP3炎症小体通路抗抑郁药效的机制研究[J].时珍国医国药,2026,37(9):1616-1623,8.基金项目
国家自然科学基金重点项目(82430126) （82430126）
国家自然科学基金面上项目(82474365,82575028) （82474365,82575028）
国家自然科学基金青年项目(82304990) （82304990）
广州市中医方证重点实验室(202102010014) （202102010014）
广东省科学技术协会青年科技人才培育计划(SKXRC2025328) （SKXRC2025328）
暨南大学黄振东中医医学研究基金(201911) （201911）
暨南大学研究生拔尖创新人才培养项目(2025CXY360) （2025CXY360）
暨南大学大学生创新创业训练计划支持项目(CX24380,CX24381) （CX24380,CX24381）
暨南大学"挑战杯"学生课外学术科技创新创业竞赛项目(202442010) （202442010）
