天津农业科学2026，Vol.32Issue(4)：1-7,7.DOI:10.3969/j.issn.1006-6500.2026.04.001
不同激素对甜瓜未授粉子房愈伤组织诱导的影响
Effects of Phytohormones on Callus Induction from Unpollinated Ovaries Culture of Melon
摘要
Abstract
To systematically investigate the effects of different phytohormones and their combinations on the callus induction from in vitro unpollinated ovary culture of melon,different concentrations of GA3,NAA,KT,2,4-D,6-BA and TDZ in individually and in combined treatments were designed,and different concentrations of coconut milk were incorporated into the medium,the effects of these factors on the callus induction rate and the morphological characteristics of callus were analyzed.The results showed that among the different combinations of GA3,TDZ,NAA,and KT in the orthogonal experiment,the order of their effects on the callus induction was NAA>KT>GA3>TDZ.The optimal concentrations were 0.5 mg·L-1NAA,0.1 mg·L-1KT,0.5 mg·L-1GA3 and 0.06 mg·L-1TDZ re-spectively.The combination of 0.5 mg·L-1NAA and 0.1 mg·L-1 KT had the best effect on callus induction,with an induction rate of 52.6%,producing callus with a dense texture and light green color.In the optimised medium containing MS+0.5 mg·L-1NAA+0.1 mg·L-1KT with the addition of 15%coconut milk,the rate of callus induction was enhanced to 68.1%,and browning rate was reduced to 7.8%.Single hormones TDZ,2,4-D,6-BA was prone to cause browning or abnormal growth of callus.In conclusion,the highly effi-cient medium for callus induction from in vitro unpollinated ovary culture of melon is MS medium supplemented with 0.5 mg·L-1 NAA,0.1 mg·L-1 KT,and 15%coconut milk.关键词
甜瓜/未授粉子房/愈伤组织/植物激素/诱导Key words
Cucumis melo L./unpollinated ovary/callus/phytohormones/induction分类
农业科技引用本文复制引用
吴峰,杨颖,陈小奇,常彩涛,陈小强..不同激素对甜瓜未授粉子房愈伤组织诱导的影响[J].天津农业科学,2026,32(4):1-7,7.基金项目
天津市大学生创新创业训练计划(202510061192) （202510061192）
