天津农业科学2026，Vol.32Issue(4)：26-32,7.DOI:10.3969/j.issn.1006-6500.2026.04.005
盐碱地纤维素分解菌筛选及其菌剂对秸秆腐解效果的研究
Screening of Cellulose-degrading Bacteria from Saline-Alkali Land and Study on the Decomposition Ef-fect of Straw by Bacterial Agent
摘要
Abstract
To address the issues of slow natural straw decomposition and limited soil fertility improvement in saline-alkali land,this study used saline-alkali soil as a microbial source to screen salt-tolerant cellulolytic bacteria,construct a composite microbial consor-tium,and evaluate its application effect on in-situ straw decomposition and return.The Congo red staining method was used to screen salt-tolerant cellulolytic bacteria,and their enzyme activities as well as salt-alkali tolerance were determined.Antagonism tests and combinatorial performance assays were conducted to construct the composite consortium.In-situ decomposition and return experiments of maize and cucumber straw were carried out to analyze the effects of the composite microbial agent on straw decomposition rate,soil organic matter,alkali-hydrolyzable nitrogen,available phosphorus,available potassium,viable bacterial count,and cucumber yield.Four bacterial strains with strong salt-tolerant cellulolytic ability were successfully screened.Among them,strain JGX10 exhibited sig-nificantly higher ratio of clear zone diameter to colony diameter(D/d)and enzyme activity than the other strains,and showed the strongest single-strain tolerance to salt and alkali,withstanding 14%NaCl and pH 11.0.Two composite microbial agents were formu-lated with different ratios of JGX10 and JGX14,among which JGXF01(1∶1)achieved the highest enzyme activity of 39.57 U·mL-1.Both composite agents effectively increased the decomposition rate of maize straw,enhanced cucumber yield,raised soil organic matter and available nitrogen,phosphorus,and potassium contents,and improved soil viable bacterial counts.JGXF01(1∶1)performed best in multiple indicators.Therefore,JGXF01(1∶1)can effectively promote straw decomposition and soil fertility improvement in saline-alkali land,providing efficient microbial resources and technical support for straw resource utilization and soil fertility enhancement in saline-alkali regions.关键词
复合菌剂/秸秆腐熟/土壤肥力/耐盐/纤维素分解菌Key words
compound microbial agents/straw decomposition/soil fertility/salt-tolerant cellulose-decomposing bacteria分类
农业科技引用本文复制引用
路垚,赵琳娜,周丽平,王静波,何宗均..盐碱地纤维素分解菌筛选及其菌剂对秸秆腐解效果的研究[J].天津农业科学,2026,32(4):26-32,7.基金项目
国家重点研发计划子课题(2024YFD1701103) （2024YFD1701103）
天津市科技计划项目(24YDTPJC00420) （24YDTPJC00420）
天津市农业科学院种业创新研究项目(2025ZYCX009) （2025ZYCX009）
