天津师范大学学报（自然科学版）2026，Vol.46Issue(1)：19-24,52,7.DOI:10.19638/j.issn1671-1114.20260104
黄芪甲苷通过调控PI3K/Akt信号通路减轻H2O2诱导的RPE细胞氧化应激与炎症损伤
Astragaloside Ⅳ attenuates H2O2-induced oxidative stress and inflammatory injury in RPE cells via activation of the PI3K/Akt signaling pathway
摘要
Abstract
To investigate the protective effects of Astragaloside Ⅳ(AS-Ⅳ)against H2O2-induced oxidative stress and in-flammatory injury in retinal pigment epithelial(RPE)cells,and to preliminarily elucidate its underlying mechanisms,an oxidative stress model was established by treating RPE cells with H2O2.The viability of cells was assessed using the CCK-8 assay,and membrane damage was evaluated by measuring LDH release.The levels of IL-6,TNF-α,and PGE2 were quantified by ELISA.Intracellular ROS accumulation was detected by ROS immunofluorescence staining,mitochondrial membrane po-tential was assessed using JC-1 staining,and the expression of PI3K/Akt pathway-related proteins and iNOS was analyzed by Western blot.The results were as follows:① CCK-8 assays showed that H2O2 treatment significantly reduced the viability of RPE cells,whereas AS-Ⅳ markedly improved the viability of H2O2-treated RPE cells in a dose-dependent manner;② H2O2 exposure significantly increased LDH release,IL-6,TNF-α,and PGE2 levels,as well as ROS accumulation and iNOS expres-sion,while AS-Ⅳ treatment significantly decreased LDH release and reduced IL-6,TNF-α,PGE2,ROS,and iNOS levels;③ H2O2 markedly decreased the red/green fluorescence ratio and reduced the relative expression of p-PI3K and p-Akt,whereas AS-Ⅳ significantly increased the red/green fluorescence ratio and promoted p-PI3K and p-Akt expression.Collectively,these findings suggest that AS-Ⅳ effectively alleviates H2O2-induced oxidative stress and inflammatory injury in RPE cells,poten-tially through reducing ROS levels,improving mitochondrial function,and activating the PI3K/Akt signaling pathway.关键词
黄芪甲苷/视网膜色素上皮细胞/氧化应激/线粒体功能/PI3K/Akt信号通路/年龄相关性黄斑变性Key words
Astragaloside Ⅳ/retinal pigment epithelium cells/oxidative stress/mitochondrial function/PI3K/Akt signaling pathway/age-related macular degeneration分类
医药卫生引用本文复制引用
洪婉冰,栾一,刘晨瑶..黄芪甲苷通过调控PI3K/Akt信号通路减轻H2O2诱导的RPE细胞氧化应激与炎症损伤[J].天津师范大学学报（自然科学版）,2026,46(1):19-24,52,7.基金项目
河南省自然科学基金资助项目(222300420284). （222300420284）
