天津中医药2026，Vol.43Issue(5)：606-612,7.DOI:10.11656/j.issn.1672-1519.2026.05.09
白藜芦醇调节RIP1/RIP3/MLKL通路对扩张型心肌病小鼠心功能的影响
Effect of resveratrol on cardiac function in mice with dilated cardiomyopathy by adjusting the RIP1/RIP3/MLKL pathway
摘要
Abstract
[Objective]To discuss the effect of resveratrol(RSV)on cardiac function in mice with dilated cardiomyopathy(DCM)by adjusting the Receptor-Interacting Serine/Threonine-Protein Kinase(RIP)1/RIP3/Mixed Lineage Kinase Domain-Like Pseudokinase(MLKL)pathway.[Methods]Male C57BL/6J mice were injected with doxorubicin to induce DCM model.Mice were stochastically separated into Control group,DCM group,DCM+RSV group,DCM+Tumor Necrosis Factor-alpha(TNF-α)group,and DCM+RSV+TNF-α group.Echocardiography was used to evaluate the cardiac function of mice in each group.ELISA was used to detect serum myocardial injury indicators of mice.HE,Masson,and DAPI propidium iodide(PI)stainings were used to observe pathological myocardial injury in mice.Immunohistochemistry was used to measure fibrosis related proteins in the myocardium of mice in each group.In addition,Western blot was used to measure the RIP1/RIP3/MLKL pathway related proteins of mice.[Results]Compared with the Control group,the DCM group showed increases in mouse heart weight,heart weight/body weight ratio,left ventricular end-diastolic diameter(LVEDD),creatine kinase-myocardial band(CK-MB),cardiac troponin t(cTn-T),B-type Natriuretic Peptide(BNP),monocyte chemoattractant protein-1(MCP-1),fibrous tissue deposition,collagen deposition,PI positivity rate,alpha-smooth muscle actin(α-SMA),collagen type Ⅲ(Collagen Ⅲ),TNF-α,p-RIP1/RIP1,p-RIP3/RIP3,and p-MLKL/MLKL,and decreases in left ventricular shortening fraction(LVSF),left ventricular ejection fraction(LVEF),and heme oxygenase-1(HO-1)(P<0.05).Compared with the DCM group or DCM+RSV+TNF-α group,the DCM+RSV group showed decreases in mouse heart weight,heart weight/body weight ratio,LVEDD,CK-MB,cTn-T,BNP,MCP-1,fibrous tissue deposition,collagen deposition,PI positivity rate,α-SMA,CollagenⅢ,TNF-α,p-RIP1/RIP1,p-RIP3/RIP3,and p-MLKL/MLKL,and increases in LVSF,LVEF,and HO-1(P<0.05);the DCM+TNF-α group showed increases in mouse heart weight,heart weight/body weight ratio,LVEDD,CK-MB,cTn-T,BNP,MCP-1,fibrous tissue deposition,collagen deposition,PI positivity rate,α-SMA,Collagen Ⅲ,TNF-α,p-RIP1/RIP1,p-RIP3/RIP3,and p-MLKL/MLKL,and decreases in LVSF,LVEF,and HO-1(P<0.05).[Conclusion]RSV effectively improves myocardial fibrosis and enhances cardiac function in DCM mice by inhibiting the RIP1/RIP3/MLKL pathway.关键词
白藜芦醇/RIP1/RIP3/MLKL通路/扩张型心肌病/心功能Key words
resveratrol/RIP1/RIP3/MLKL pathway/dilated cardiomyopathy/cardiac function分类
医药卫生引用本文复制引用
饶锡东,朱宏飞,武美娇..白藜芦醇调节RIP1/RIP3/MLKL通路对扩张型心肌病小鼠心功能的影响[J].天津中医药,2026,43(5):606-612,7.基金项目
湖北省卫生健康科技项目(WJ2025ZH025) （WJ2025ZH025）
湖北省教育厅科学技术研究计划项目(B2023096). （B2023096）
