西北农业学报2026，Vol.35Issue(5)：809-814,6.DOI:10.7606/j.issn.1004-1389.2026.05.002
O型FMDV持续感染牛体内的病毒分布特征
Characterization of Virus Distribution in Persistently Infected Cattle with FMDV Type O
摘要
Abstract
Persistent infection with the Foot-and-Mouth Disease Virus(FMDV)in cattle is a current challenge in the prevention and control of Foot-and-Mouth Disease(FMD).In this study,cattle that had undergone post-vaccination challenge protection tests under laboratory conditions were used as the experimental animal model,under laboratory conditions,cattle that had undergone post-vaccination challenge protection tests were used as the experimental animal model.Individuals that showed no clinical symptoms 28 days post-challenge but tested positive for viral nucleic acid in oropharyngeal(O/P)fluid were selected as subjects foto investigater studying persistent infection.The distribution characteristics of FMDV in various tissues of persistently infected cattle werewas systematically ana-lyzed using quantitative real-time PCR(qRT-PCR)to detect viral nucleic acid load(Ct value)in tis-sues,Haematoxylin and Eosin staining to observe histopathological changes,and immunohistochemical staining to localize viral antigens.The qRT-PCR results revealedshowed that FMDV distribution in persistently infected cattle exhibited tissue specificity,exhibits tissue specificity,with viral nucleic acids predominantly enriched in the palatine tonsils,nasopharyngeal tonsils,and O/P tissues.The re-sults from H&E staining and immunohistochemical staining showed that pathological changes such as partial lymphocyte necrosis and hemorrhage were observed in the tonsils and O/P tissues,along with weakly positive signals for viral antigens in these tissues.关键词
口蹄疫病毒/荧光定量PCR/持续性感染/病毒分布Key words
Foot-and-Mouth Disease Virus/qRT-PCR/Persistent infection/Viral distribution引用本文复制引用
王锴,张智慧,张莹,丁耀忠,郭慧琛,孙世琪,尹双辉,马小军..O型FMDV持续感染牛体内的病毒分布特征[J].西北农业学报,2026,35(5):809-814,6.基金项目
"十四五"重点研发计划(2021YFD1800303) （2021YFD1800303）
中国-非洲伙伴研究所交流项目([2023]14号). Key Research and Development Project of the 14th Five-Year Plan(No.2021YFD1800303) （[2023]14号）
China-Africa Partner Institution Exchange Project(No.[2023]14). （No.[2023]14）
