温度与基质处理对太白贝母种子后熟及胚率的影响
Effects of Temperature and Substrate on After-Ripening and the Rate of Embryo Development in Fritillaria taipaiensis Seeds
摘要
Abstract
This study examines how temperature and substrate affect embryo development and the proportion of mature embryos in Fritillaria taipaiensis seeds,to provide a theoretical and technical basis for optimizing dormancy-breaking protocols and standardizing cultivation.F.taipaiensis seeds were subjected to four temperature regimes(5,10,15,20℃)and five substrates(sandy soil,river sand,peat soil,sandy soil-peat mix,and river sand-peat mix).Seed-substrate mixtures(v/v=1∶4)were stratified at 5-20℃ under 50％-60％(w/w)moisture content,and sampled every 10 days.Embryo maturity rates were determined microscopically to identify the optimal temperature,substrate,and duration for after-ripening of F.taipaiensis seeds.The results demonstrated that low-temperature stratification(5℃ and 10℃)in combination with organic(peat soil)or highly aerated substrates(sandy soil and river sand)significantly accelerated embryo development of F.taipaiensis seeds.At 5℃,80 days of stratification on single substrates-sandy soil,river sand,and peat soil-pro-duced embryo rates well above the 100％reference(139.36％,160.00％and 130.33％,respective-ly),whereas the two mixed substrates(sandy soil+peat soil and river sand+peat soil)gave signif-icantly lower values(95.24％and 88.00％).At 10℃,river sand alone again achieved the highest embryo rate(120.00％),while the river-sand/peat-soil mixture recorded the lowest(88.00％).High-temperature stratification(15℃ and 20℃)markedly inhibited embryo development.After 80 days,the highest embryo rate at 15℃ was 75.00％(sandy soil),whereas at 20℃ it fell to just 44.44％(river sand).Low-temperature stratification at 5℃,combined with either organic(peat soil)or highly aerated substrates(sandy soil and river sand),effectively breaks seed dormancy,markedly accelerates embryo development,and shortens the after-ripening period.These findings provide critical parameters and theoretical guidance for standardizing seed pretreatment protocols in the artificial cultivation of Fritillaria taipaiensis.关键词
太白贝母/种子/萌发特性/温度/基质Key words
Fritillaria taipaiensis/Seeds/Germination characteristics/Temperature/Substrate引用本文复制引用
徐胜,杨冰月,颜永刚,张岗,周静,强毅,陆博,张小飞,彭亮..温度与基质处理对太白贝母种子后熟及胚率的影响[J].西北农业学报,2026,35(5):867-873,7.基金项目
秦巴山区及吕梁山区特色中药材生态种植模式创建与示范(2023YFD1600402) （2023YFD1600402）
国家重点研发计划(2024YFC3506400) （2024YFC3506400）
陕西省科技厅社发重点项目(No.2024SF-GJHX-05) （No.2024SF-GJHX-05）
陕西省中医药管理局中医药全省性专款专项(2021-QYZL-02) （2021-QYZL-02）
中央本级重大增减支项目(2060302). Development and Demonstration of Eco-Cultivation Models for Characteristic Me-dicinal Plants in Qinba and Lüliang Mountainous Areas(No.2023YFD1600402) （2060302）
National Key R&D Program of China(No.2024YFC3506400) （No.2024YFC3506400）
Key Project for Social Development of Shaanxi Provincial Science and Technology Department(No.2024SF-GJHX-05) （No.2024SF-GJHX-05）
Provincial Special Fund Project of Shaanxi Provincial Administration of Chinese Medicine:Research on Cultivation Guidelines for Qin-re-gion Medicinal Herbs(No.2021-QYZL-02) （No.2021-QYZL-02）
Central-Level Major Special Project for Budget Revenue and Expenditure Adjustments(No.2060302). （No.2060302）
