西北农业学报2026，Vol.35Issue(5)：874-879,6.DOI:10.7606/j.issn.1004-1389.2026.05.009
湿软生物样品SEM预处理方法的新策略:一种基于RTILs的简便经济之法
New Strategy for Pretreatment of Hydrated Soft Biological Samples for SEM:a Simple and Cost-effective Approach Based on RTILs
摘要
Abstract
To demonstrate the utility of the RTIL-based SEM pretreatment method for hydrated soft biological specimens,this study replaced the conventional time-consuming,labor-intensive,and expen-sive SEM sample preparation with a simple droplet application and blotting treatment.The results showed that although the RTIL-based method did not achieve perfect preservation of morphology,it produced results comparable to those of conventional methods.Specifically,it demonstrated superior structural fidelity in preserving spore quantity,spore chains,leaf trichomes,and epidermal cells,provi-ding a more realistic representation of biological structures than traditional SEM pre-treatment.Addi-tionally,its electrical conductivity was comparable to that of conventional methods.The developed RTIL-based approach shows great potential as an alternative to conventional SEM pretreatment,offer-ing advantages in cost,simplicity,and efficiency,while maintaining image quality nearly identical to that of traditional protocols.Notably,it outperforms standard methods in preserving critical structural features such as the bead-like morphology of Alternaria spore chains and cellular turgor.关键词
扫描电子显微镜/室温离子液体/1-乙基-3-甲基-1H-咪唑-3-鎓四氟硼酸盐/链格孢菌/烟草叶片Key words
Scanning Electron Microscopy(SEM)/Room Temperature Ionic Liquids(RTILs)/1-Ethyl-3-methylimidazolium tetrafluoroborate([EMIM][BF4])/Alternaria Nees/Tobacco leaves引用本文复制引用
何晓华,曹予凡,郝思嘉,赵涵钰,张璟,刘斌..湿软生物样品SEM预处理方法的新策略:一种基于RTILs的简便经济之法[J].西北农业学报,2026,35(5):874-879,6.基金项目
陕西省自然科学基础研究计划(2023-JC-QN-0235) （2023-JC-QN-0235）
西北农林科技大学实验技术研究与实验室管理创新类项目(SY20250101). Natural Science Basic Research Program of Shaanxi Province(No.2023-JC-QN-0235) （SY20250101）
Innovation Project of Experimental Technology Research and Laboratory Management of Northwest A&F University(No.SY20250101). （No.SY20250101）
