陕西科技大学学报2026，Vol.44Issue(3)：80-91,98,13.
高活性功能微生物定向进化研究进展
Research progress on directed evolution of highly active functional microorganisms
摘要
Abstract
During the industrial application of functional microorganisms,phenotypic degrada-tion such as reduced growth rate and decreased metabolic product synthesis capacity often oc-curs,severely affecting industrial outcomes and product quality.Directed evolution technolo-gy,which simulates natural evolutionary processes by combining artificially induced genetic variation with targeted screening,serves as a core strategy for enhancing strain stability and performance.However,its application in the field of functional microorganisms remains limit-ed.To promote the adoption of directed evolution in the functional microorganism industry,this article reviews the main causes of strain degradation in industrial settings and summari-zes the commonly used physical,chemical,and biological methods of directed evolution.By examining the molecular mechanisms,application outcomes,and limitations of these meth-ods,this review aims to provide theoretical support for maintaining desirable traits in func-tional microorganisms and to advance the widespread application of directed evolution tech-nology in the microbial industry.关键词
功能微生物/菌株衰退/定向进化/诱变/产业化应用Key words
functional microorganisms/strain degeneration/directed evolution/mutagenesis/industrial applications分类
轻工纺织引用本文复制引用
高磊,吴清平,陈玲,赵昕宇,李滢..高活性功能微生物定向进化研究进展[J].陕西科技大学学报,2026,44(3):80-91,98,13.基金项目
国家重点研发计划项目(2023YFF1000303) （2023YFF1000303）
广东省自然科学基金项目(2025A1515012225) （2025A1515012225）
广东省科学院项目(2022GDASZH-2022010101) （2022GDASZH-2022010101）
