现代中西医结合杂志2026，Vol.35Issue(6)：745-751,7.DOI:10.3969/j.issn.1008-8849.2026.06.003
补肾活血方对强直性脊柱炎小鼠的保护作用及对SIRT1表达的影响
Protective effect of prescription for tonifying kidney and activating blood on mice with ankylosing spondylitis and its influence on the expression of SIRT1
摘要
Abstract
Objective It is to observe the anti-inflammatory effect and its mechanism of prescription for tonifying kidney and activating blood(PTKAB)in the treatment of ankylosing spondylitis(AS)in mice.Methods ①Forty DBA/1 male mice were randomly divided into model group,low dose PTKAB group,high dose PTKAB group and celecoxib group,an-other 10 C57BL/6 mice were selected as the control group.The low dose PTKAB group and high dose PTKAB group were given PTKAB 12 g/kg,24 g/kg via gavage,respectively,while the model group and control group were given the same a-mount of normal saline via gavage,all once daily,continuously treated for 4 weeks.The degree score of joint swelling was evaluated,the histopathological morphology of the achilles tendon tissue was observed by HE staining,the serum levels of interleukin(IL)-6,IL-1β,IL-23,IL-17αand tumor necrosis factor-α(TNF-α)were measured by ELISA,and the protein expressions of silent information regulator 1(SITR1),CD86,and inducible nitric oxide synthase(iNOS)in achilles ten-don tissue were detected by Western blot.②THP-1 cells were taken and cultured in vitro,4 groups were set up in the ex-periment.The control group was cultured with 20 nmol/L PMA,the lipopolysaccharide(LPS)group was cultured with 20 nmol/L PMA+100 ng/mL LPS,the PTKAB group was cultured with 20 nmol/L PMA+100 ng/mL LPS+medicated ser-um containing 10%PTKAB,the PTKAB+si-SIRT1 group was cultured with si-SIRT1 plasmid transfection+20 nmol/L PMA+100 ng/mL LPS+medicated serum containing10%PTKAB.After24 hours of treatment,the protein expressions of SIRT1 and iNOS in the cells were detected by Western blot,and the expression of iNOS in the cells was detected by im-munofluorescence.Results ①Compared with the model group,the joint swelling scores and serum levels of IL-6,IL-1β,IL-23,IL-17α and TNF-α of mice in the medicated groups were significantly reduced(all P<0.05),and the inflammato-ry cell infiltration in achilles tendon tissue was significantly alleviated;the relative protein expressions of SITR1 in achilles tendon tissue were significantly increased(all P<0.05),while the relative protein expressions of CD86 and iNOS in achil-les tendon tissue were significantly decreased in the low dose and high dose groups of PTKAB(all P<0.05).②Compared with the blank group,the relative protein expression of SITR1 was significantly reduced in the LPS group(P<0.05),while the relative protein expression of iNOS and the relative fluorescence intensity of iNOS expression were both significant-ly increased(both P<0.05).Compared with the LPS group,the relative protein expression of SITR1 was significantly in-creased in the PTKAB group(P<0.05),while the relative protein expression of iNOS and the relative fluorescence inten-sity of iNOS expression were both significantly decreased(both P<0.05).Compared with the PTKAB group,the relative protein expression of SITR1 was significantly reduced in the PTKAB+si-SIRT1 group(P<0.05),while the relative pro-tein expression of iNOS and the relative fluorescence intensity of iNOS expression were both significantly increased(both P<0.05).Conclusion PTKAB can inhibit inflammation through regulation of SITR1-mediated polarization of macrophage M1,thus to improve joint swelling and slow down the progression of AS.关键词
补肾活血方/强直性脊柱炎/沉默信息调节因子1/关节肿胀/炎症反应/巨噬细胞极化Key words
prescription for tonifying kidney and activating blood/ankylosing spondylitis/silent information regulator 1/joint swelling/inflammatory response/macrophage polarization分类
医药卫生引用本文复制引用
杨晔颖,朱竹菁,龚蓓,张令悦,曲环汝..补肾活血方对强直性脊柱炎小鼠的保护作用及对SIRT1表达的影响[J].现代中西医结合杂志,2026,35(6):745-751,7.基金项目
上海中医药大学科技发展基金项目(23KFL072) （23KFL072）
上海市科技创新计划项目(23Y11920400) （23Y11920400）
