眼科新进展2026，Vol.46Issue(5)：341-346,6.DOI:10.13389/j.cnki.rao.2026.0061
黄精多糖对高糖诱导的晶状体上皮细胞内质网应激及凋亡的影响
Effect of Polygonatum sibiricum polysaccharide on endoplasmic reticulum stress and apoptosis of lens epithelial cells induced by high glucose
摘要
Abstract
Objective To investigate the effects of Polygonatum sibiricum polysaccharide(PSP)on endoplasmic re-ticulum stress(ERS)and apoptosis of lens epithelial cells(LECs)induced by high glucose(HG).Methods SRA01/04 cells were divided into the Control group,HG group,and HG+PSP group.Cell viability was measured by the Cell Counting Kit-8(CCK-8)assay to determine the optimal concentration of PSP.An in vitro rat lens culture model was established to observe the effect of PSP on lens opacity.The expression levels of ERS-related proteins,including phosphorylated protein kinase-like endoplasmic reticulum kinase(p-PERK),protein kinase-like endoplasmic reticulum kinase(PERK),transcrip-tion factor C/EBP homologous protein(CHOP),and glucose-regulated protein 78(GRP78),as well as apoptosis-related proteins,including B-cell lymphoma/leukemia-2(Bcl-2)and Bcl-2-associated X protein(Bax)were detected by Western blot.The expression and distribution of GRP78 were measured by immunofluorescence staining,and cell apoptosis was quantified by TUNEL assay.Results CCK-8 assay showed that compared with the Control group,cell viability in the HG group significantly decreased(P＜0.001).Compared with the HG group,cell viability increased to varying degrees after in-tervention with different concentrations of PSP,with the most significant increase observed at a PSP concentration of 25.00μmol·L-1(P＜0.01).In the in vitro lens culture,the percentage of lens opacity in the HG group was significantly higher than that in the Control group(P＜0.001),while it decreased significantly in the HG+PSP group compared with the HG group(P＜0.01).Western blot results revealed that compared with the Control group,the expression levels of p-PERK/PERK,GRP78,and CHOP proteins were up-regulated,while the Bcl-2/Bax ratio was down-regulated in the HG group(all P＜0.05).Compared with the HG group,the expression levels of the aforementioned ERS-related proteins significantly de-creased,while the Bcl-2/Bax ratio significantly increased in the HG+PSP group(all P＜0.05).Immunofluorescence stai-ning showed that the fluorescence intensity of GRP78 in the HG group was significantly higher than that in the Control group(P＜0.01),while it was significantly reduced in the HG+PSP group compared with the HG group(P＜0.01).TUNEL assay demonstrated that the apoptosis level in the HG group increased compared with the Control group,which was effectively at-tenuated by PSP treatment(all P＜0.05).Conclusion PSP can delay the progression of lens opacity by inhibiting HG-in-duced ERS and apoptosis in LECs.关键词
糖尿病性白内障/黄精多糖/晶状体上皮细胞/内质网应激/凋亡Key words
diabetic cataract/Polygonatum sibiricum polysaccharide/lens epithelial cells/endoplasmic reticulum stress/apoptosis分类
医药卫生引用本文复制引用
王融,李鹏飞,管怀进,陈威,季敏..黄精多糖对高糖诱导的晶状体上皮细胞内质网应激及凋亡的影响[J].眼科新进展,2026,46(5):341-346,6.基金项目
国家自然科学基金项目(编号:82571192,82571190) （编号:82571192,82571190）
