疑难病杂志2026，Vol.25Issue(5)：519-524,6.DOI:10.3969/j.issn.1671-6450.2026.05.002
非小细胞肺癌患者血清HSPH1及TRIM11 mRNA、CLCA2 mRNA水平与淋巴结转移及预后的关系分析
Analysis of the relationship between serum HSPH1,TRIM11,and CLCA2 levels and lymph node metastasis and prognosis in patients with non-small cell lung cancer
摘要
Abstract
Objective To analyze the relationship between serum heat shock protein H1(HSPH1),tripartite motif containing 11(TRIM11),and calcium-activated chloride channel protein 2(CLCA2)levels and lymph node metastasis and prognosis in patients with non-small cell lung cancer(NSCLC).Methods The NSCLC group comprised 80 patients with NSCLC who received treatment in the Department of Respiratory Medicine at the 902nd Hospital of the Joint Logistics Sup-port Force of the People's Liberation Army between February 2022 and February 2024.Based on the presence of lymph node metastasis,patients were categorized into a metastatic subgroup(34 cases)and a non-metastatic subgroup(46 cases).Based on prognosis,they were further divided into a survival subgroup(52 cases)and a mortality subgroup(28 cases).Additionally,70 patients with benign pulmonary lesions admitted to the hospital during the same period were recruited to form the benign group,while 80 volunteers from the hospital's health screening program served as the healthy control group.Serum HSPH1 levels were determined using ELISA,while TRIM11 mRNA and CLCA2 mRNA levels were assessed via qRT-PCR.Relative risk analysis was used to evaluate the impact of serum HSPH1,TRIM11 mRNA,and CLCA2 mRNA levels on lymph node metastasis in NSCLC patients.ROC curve analysis was used to evaluate the prognostic predictive value of serum HSPH1,TRIM11 mRNA,and CLCA2 mRNA in patients with non-small cell lung cancer.Results Serum HSPH1 and TRIM11 mRNA levels progressively increased across the healthy,benign,and NSCLC groups,while serum CLCA2 mRNA levels pro-gressively decreased(F/P=568.905/＜0.001,595.132/＜0.001,309.738/＜0.001).Compared with the non-metastatic subgroup,the metastatic subgroup exhibited significantly elevated serum HSPH1 and TRIM11 mRNA levels and significantly reduced serum CLCA2 mRNA levels(t/P=3.067/0.003,3.866/＜0.001,4.192/＜0.001).Compared with the survival subgroup,the mor-tality subgroup exhibited significantly elevated serum HSPH1 and TRIM11 mRNA levels and significantly reduced serum CLCA2 mRNA levels(t/P=7.818/＜0.001,7.191/＜0.001,7.992/＜0.001).Patients with elevated serum HSPH1 and TRIM11 mRNA levels had a 2.171-fold and 2.273-fold increased risk of lymph node metastasis,respectively,compared with those with low levels(x2/P=7.758/0.005,8.203/0.004).Patients with low serum CLCA2 mRNA levels had a 3.156-fold increased risk of lymph node metastasis compared with those with high levels(x2/P=14.238/＜0.001).The AUC values for predicting poor prog-nosis in NSCLC patients using serum HSPH1,TRIM11 mRNA,CLCA2 mRNA individually,and their combination were 0.811,0.782,0.817,and 0.918,respectively,with the combined predictive value significantly surpassing that of individual markers(Z=2.173,2.103,2.165;P=0.030,0.035,0.030).Conclusion NSCLC patients exhibit elevated serum HSPH1 and TRIM11 levels and reduced serum CLCA2 levels,which correlate with lymph node metastasis and survival prognosis.Furthermore,the com-bined assessment of these three markers demonstrates favorable predictive value for the prognosis of NSCLC patients.关键词
非小细胞肺癌/热休克蛋白H1/三结构域蛋白11/钙激活性氯离子通道蛋白2/淋巴结转移/预后Key words
Non-small cell lung cancer/Heat shock protein H1/Tripartite motif containing 11/Calcium-activated chloride channel protein 2/Lymph node metastasis/Prognosis分类
医药卫生引用本文复制引用
张华,瞿霏霏,范铁兰,胡宗涛,娄志霞..非小细胞肺癌患者血清HSPH1及TRIM11 mRNA、CLCA2 mRNA水平与淋巴结转移及预后的关系分析[J].疑难病杂志,2026,25(5):519-524,6.基金项目
2022年安徽省卫生健康科研项目(AHWJ2022b079) 2022 Anhui Provincial Health Science Research Project(AHWJ2022b079) （AHWJ2022b079）
