空军军医大学学报2026，Vol.47Issue(5)：651-659,9.DOI:10.13276/j.issn.2097-1656.2026.05.004
硫酸二甲酯通过氧化应激-线粒体凋亡通路诱导人支气管上皮细胞急性损伤的机制研究
Mechanism of dimethyl sulfate-induced acute injury in human bronchial epithelial cells via oxidative stress-mitochondrial apoptosis pathway
摘要
Abstract
Objective To investigate the acute toxic effects of dimethyl sulfate(DMS)on human bronchial epithelial cells(BEAS-2B)and its underlying mechanisms.Methods An in vitro model of acute lung injury(ALI)was established by treating BEAS-2B cells with DMS.Cell viability after DMS exposure at different concentrations and time points was assessed using the CCK-8 assay.Changes in cell morphology were observed under an optical microscope.The apoptosis rate was measured by Annexin V/PI double staining.Mitochondrial membrane potential changes,malondialdehyde(MDA)content,intracellular total reactive oxygen species(ROS)and mitochondrial reactive oxygen species(mtROS)levels,reduced glutathione(GSH)and oxidized glutathione(GSSG)content,as well as total superoxide dismutase(SOD)and catalase(CAT)activity were detected using corresponding assay kits.Results Compared to the control group,the relative cell viability in DMS-treated groups significantly decreased(P<0.01)and progressively declined with increasing exposure time and concentration(P<0.01).The apoptosis rate significantly increased(P<0.01),and the mitochondrial transmembrane potential was markedly reduced(P<0.01).Intracellular MDA content initially increased without statistical significance but rose rapidly at later stages(P<0.01),showing a significant time-and dose-dependent effect.Both intracellular total ROS and mtROS levels significantly increased(P<0.01).In the early exposure stage,GSH content significantly increased while GSSG rose slowly.With prolonged exposure,GSH content decreased rapidly,while GSSG content increased sharply(P<0.01).Under low-concentration DMS exposure,total SOD activity increased over time but was inhibited at high concentrations.The change in CAT activity showed an opposite trend to total SOD(P<0.01).Conclusion An in vitro model of DMS-induced acute injury in BEAS-2B cells is successfully established.This model can be used to simulate the pathological process of ALI in humans caused by short-term,high-dose inhalation of DMS.Acute DMS exposure significantly reduces cell viability and induces severe apoptosis.The mechanism may be related to mitochondrial membrane potential disruption,excessive ROS generation,disruption of intracellular redox balance,and severe oxidative damage,ultimately leading to apoptosis and loss of cell viability.关键词
硫酸二甲酯/活性氧/线粒体/氧化应激/超氧化物歧化酶/过氧化氢酶/谷胱甘肽/丙二醛Key words
dimethyl sulfate/reactive oxygen species/mitochondria/oxidative stress/superoxide dismutase/catalase/glutathione/malondialdehyde分类
医药卫生引用本文复制引用
陈潇湘,于卫华,刘江正,海春旭,孔德钦,刘瑞,张倩,李璐迪,柳冰洁,吴昊,曹猛,安广洲..硫酸二甲酯通过氧化应激-线粒体凋亡通路诱导人支气管上皮细胞急性损伤的机制研究[J].空军军医大学学报,2026,47(5):651-659,9.基金项目
陕西省重点研发计划项目(2023-YBSF-297) （2023-YBSF-297）
