空军军医大学学报2026，Vol.47Issue(5)：660-665,672,7.DOI:10.13276/j.issn.2097-1656.2026.05.005
游离DNA在硫芥致急性肺损伤中的早期诊断价值
Early diagnostic value of cell-free DNA in sulfur mustard-induced acute lung injury
摘要
Abstract
Objective To investigate the diagnostic value of cell-free DNA(cf-DNA)in acute lung injury induced by sulfur mustard(SM).Methods C57BL/6 mice were exposed to 80 mL/L of the SM simulator 2-chloroethyl ethyl sulfide(CEES)via nebulization inhalation.The effects of CEES on mouse body mass and lung-to-body weight ratio were examined.HE and TUNEL staining were used to assess changes in lung tissue structure and pulmonary cell apoptosis,respectively.Pulmonary function was evaluated using a whole-body plethysmography system.Protein concentration and cell count in bronchoalveolar lavage fluid(BALF)were measured by BCA protein assay and flow cytometry,respectively.Hematological parameters were analyzed using an automated hematology analyzer.Inflammatory factor concentrations in serum and BALF were determined using ELISA kits.Total cf-DNA concentration and cf-mtDNA copy number in serum and BALF were measured using a microspectrophotometer and a PCR instrument,respectively.Results Compared with the control group,the CEES-exposed group showed reduced body mass(P<0.01),disrupted lung tissue structure,increased lung-to-body weight ratio(P<0.01),decreased lung function(P<0.05),no significant differences in BALF cell count or protein concentration,no obvious pulmonary cell apoptosis by TUNEL staining,and normal hematological parameters.Serum and BALF levels of inflammatory factors IL-1β and TNF-α were significantly elevated(P<0.01).Serum total cf-DNA concentration showed no change,while serum cf-mtDNA copy number decreased(P<0.05).BALF total cf-DNA concentration significantly increased,and cf-mtDNA copy number also increased(P<0.05).Conclusion In SM-induced acute lung injury,before significant cell apoptosis or marked abnormalities in hematological parameters occur,BALF total cf-DNA concentration significantly increases,and cf-mtDNA copy numbers in serum and BALF change.These changes may serve as early detection indicators for SM exposure.关键词
硫芥/急性肺损伤/游离DNA/早期诊断/炎症/肺泡灌洗液/液体活检/2-氯乙基乙基硫醚Key words
sulfur mustard/acute lung injury/cell-free DNA/early diagnosis/inflammation/bronchoalveolar lavage fluid/liquid biopsy/2-chloroethyl ethyl sulfide分类
医药卫生引用本文复制引用
樊振鹏,刘江正,孔德钦,李文丽,师敏婕,郭晓洁,李佳威,张瑜娇,高天,王常艳,曹猛,于卫华..游离DNA在硫芥致急性肺损伤中的早期诊断价值[J].空军军医大学学报,2026,47(5):660-665,672,7.基金项目
国家自然科学基金青年科学基金(32100996) （32100996）
国家自然科学基金面上项目(82173544,82373603) （82173544,82373603）
陕西省重点研发计划项目(2023-YBSF-297) （2023-YBSF-297）
