空军军医大学学报2026，Vol.47Issue(5)：673-681,9.DOI:10.13276/j.issn.2097-1656.2026.05.007
CIRBP通过抑制星形胶质细胞A1型极化改善低氧暴露小鼠学习记忆功能
CIRBP ameliorates hypoxia-induced learning and memory deficits in mice by suppressing astrocyte A1 polarization
摘要
Abstract
Objective To investigate whether cold-inducible RNA-binding protein(CIRBP)exerts a protective effect on the learning and memory function of mice exposed to hypoxia by regulating astrocyte A1 polarization in the hippocampus.Methods C57BL/6 mice were randomly divided into 10 groups:(1)normoxic group;(2)normoxia+Lv-NC group(stereotactic injection of Lv-NC negative control lentivirus);(3)normoxia+0.154 mol/L Saline control group(stereotactic injection of normal saline);(4)normoxia+overexpression of CIRBP group(stereotactic injection of CIRBP overexpression lentivirus);(5)normoxia+Zr17-2 group(receptor agonist Zr17-2 intervention);(6)hypoxia group;(7)hypoxia+Lv-NC group(stereotactic injection of Lv-NC negative control lentivirus after hypoxia exposure);(8)hypoxia+0.154 mol/L Saline control group(first exposed to hypoxic environment,and then stereotactic injection of normal saline);(9)hypoxia+overexpression of CIRBP group(after hypoxia exposure,stereotactic injection of CIRBP overexpression lentivirus);(10)hypoxia+Zr17-2 group(after hypoxia exposure,the receptor agonist Zr17-2 was given).All mice were routinely fed after injection,and their learning and memory functions were subsequently detected by Morris water maze and novel object recognition experiments.Western blotting was used to detect the overexpression level of CIRBP in astrocytes in the hippocampus.Immunofluorescence histochemical staining and qRT-PCR were used to detect polarization-related phenotypic changes related to astrocytes A1 in the hippocampus.Results Compared with the normoxic group,the expression level of CIRBP protein in the hippocampus of mice in the hypoxia group was significantly decreased(P<0.01),the expression level of astrocyte A1 polarization in the hippocampus was significantly increased(P<0.01),and the learning and memory ability of mice was significantly decreased(P<0.01).After overexpression of CIRBP or the use of the receptor agonist Zr17-2,compared with the control mice,the expression level of CIRBP protein in the hippocampus of the mice in the treatment group was significantly increased(P<0.01),while the expression level of astrocyte A1 polarization was reduced(P<0.05,P<0.01),and the learning and memory ability of the mice was significantly restored(P<0.05,P<0.01).Conclusion CIRBP protects the learning and memory functions of mice exposed to hypoxia by regulating astrocyte A1 polarization.关键词
低氧/学习记忆/海马/星形胶质细胞/冷诱导RNA结合蛋白/A1型极化/小鼠/认知障碍Key words
hypoxia/learning and memory/hippocampus/astrocytes/cold-inducible RNA-binding protein/A1 polarization/mice/cognitive impairment分类
医药卫生引用本文复制引用
霍晓东,赵芳,王俣辰,王博玄,侯锦超,官瑞丽,周杨,陈景元,邹远康..CIRBP通过抑制星形胶质细胞A1型极化改善低氧暴露小鼠学习记忆功能[J].空军军医大学学报,2026,47(5):673-681,9.基金项目
国家自然科学基金青年科学基金(82302116) （82302116）
陕西省自然科学基础研究项目(2024JC-YBQN-0865) （2024JC-YBQN-0865）
空军军医大学领航行动新飞计划项目(2023-rcjfzyk) （2023-rcjfzyk）
