空军军医大学学报2026，Vol.47Issue(5)：698-706,9.DOI:10.13276/j.issn.2097-1656.2026.05.010
补阳还五汤通过抑制杏仁核神经炎症与突触过度激活改善小鼠焦虑样行为
Buyang Huanwu Decoction ameliorates anxiety-like behaviors in mice by inhibiting neuroinflammation and excessive synaptic activation in the amygdala
摘要
Abstract
Objective To investigate the ameliorative effect of Buyang Huanwu Decoction(BHD)on stress-induced anxiety-like behaviors in mice,as well as its underlying mechanisms based on neuroinflammation and synaptic plasticity in the amygdala(Amy).Methods Forced swimming stress was used to establish a mouse anxiety model.Male C57BL/6J mice were randomly divided into seven groups:normal control group,normal control plus high-dose BHD group,model group,model plus low/medium/high-dose BHD groups,and model plus diazepam positive control group.Anxiety-like behaviors were evaluated by the open field test and elevated plus maze test.Western blotting and qRT-PCR techniques were employed to detect the expression levels of microglial marker(Iba1),astrocyte marker(GFAP),inflammatory factors(IL-6,IL-1β,TNF-α),and synaptic plasticity-related proteins(PSD95,Drebrin,GluA1 and its phosphorylation sites S831/S845,GluN2B and its phosphorylation sites S1303/T1472)in the Amy of mice.Results Compared with the control group,mice in the model group exhibited significant anxiety-like behaviors(P<0.01).Meanwhile,the protein expressions of Iba1 and GFAP,as well as the mRNA expressions of IL-6,IL-1β and TNF-α in the Amy were significantly upregulated(P<0.01);the expressions of postsynaptic proteins(PSD95,Drebrin),glutamate receptor subunits(GluA1,GluN2B)and their key phosphorylation levels were also significantly increased(P<0.05,P<0.01).Pretreatment with BHD dose-dependently reversed the above behavioral abnormalities,with the best effect observed in the high-dose group(P<0.01),and simultaneously inhibited the activation of glial cells,the release of inflammatory factors,and the abnormally high expression of synaptic plasticity-related proteins in the Amy.Conclusion BHD may ameliorate stress-induced anxiety-like behaviors in mice by inhibiting the activation of glial cells in the Amy,alleviating local neuroinflammatory responses,and regulating the expression of synaptic plasticity-related proteins.This study provides reliable experimental evidence for the clinical application of BHD in the treatment of anxiety-related diseases.关键词
补阳还五汤/焦虑/应激/杏仁核/神经炎症/突触可塑性/神经胶质细胞/谷氨酸受体Key words
Buyang Huanwu Decoction/anxiety/stress/amygdala/neuroinflammation/synaptic plasticity/glial cells/glutamate receptors分类
医药卫生引用本文复制引用
鲁贝宁,姜永莉,颜雨暄,马雪,岳姣,郭青娟,王欣赏,刘水冰..补阳还五汤通过抑制杏仁核神经炎症与突触过度激活改善小鼠焦虑样行为[J].空军军医大学学报,2026,47(5):698-706,9.基金项目
国家自然科学基金(82571721,82404591,82571359) （82571721,82404591,82571359）
陕西省科技创新团队计划项目(2023-CX-TD-63) （2023-CX-TD-63）
空军军医大学航空医学重点问题科技攻关项目(2024GJJH03-02) （2024GJJH03-02）
空军军医大学快速响应课题(2023KXKT064) （2023KXKT064）
