园艺学报Issue(4)：1013-1024,12.DOI:10.16420/j.issn.0513-353x.2025-0960
枳丛枝菌根特异性脂质分配模式对干旱胁迫的响应
Arbuscular Mycorrhizal Fungi-Specific Lipid Allocation Patterns in Citrus Mycorrhizae in Response to Drought Stress
摘要
Abstract
With trifoliate orange[Poncirus trifoliata(L.)Raf.]as plant materials inoculated with arbuscular mycorrhizal fungus(AMF)Rhizophagus irregularis DAOM197198 or not,this study investigated the allocation patterns of arbuscular mycorrhizal fungi(AMF)-specific lipids in response to drought stress under well watered(soil water content 18%)or drought stressed(soil water content 10%)conditions,in which the lipid contents in roots and soils and the expression levels of related genes were determined.Results showed that AMF significantly promoted plant growth,with the biomass increasing by 49.09%and 29.76%respectively after 1(weak stress)and 4(strong stress)weeks of drought.Drought significantly inhibited AMF colonization,and significantly reduced the AMF-specific lipid(C16∶1ω5)content as well.The specific phospholipid fatty acid(PLFA-C16∶1ω5)contents decreased by 18.59%and 61.52%respectively after 1(weak stress)and 4(strong stress)weeks of drought,while the specific neutral lipid fatty acid(NLFA-C16∶1ω5)contents decreased by 54.08%and 46.05%,indicating the greater effect of strong stress on PLFA-C16∶1ω5 than that of weak stress.Under well watered condition,NLFA-C16∶1ω5 mainly distributed intraradically with drought promoting it to distribute outwards in soils,while PLFA-C16∶1ω5 mainly distributed extraradically with drought further increasing its proportion in soils.Moreover,drought inhibited the expression of genes related to specific lipid synthesis and transport(PtFatM,PtRAM2,PtSTR,PtSTR2),P transporter PtPT4 and sugar transporter PtSWEET2.In conclusion,considering the close relations of PLFA-C16∶1ω5 with arbuscule(hyphae),and NLFA-C16∶1ω5 with spores(vesicles),it is acceptable that AMF respond to drought stress by differentially allocating lipids based on function(PLFA vs.NLFA)and spatial distribution(intraradical vs.extraradical).关键词
枳/干旱胁迫/丛枝菌根真菌/AMF特异性脂肪酸/C16∶1ω5/脂质分配Key words
Poncirus trifoliata/drought stress/arbuscular mycorrhizal fungi/AMF-specific fatty acids/C16∶1ω5/lipid allocation分类
农业科技引用本文复制引用
张微,尹喜龙,冯曾威,刘晓迪,周杨,朱红惠,姚青..枳丛枝菌根特异性脂质分配模式对干旱胁迫的响应[J].园艺学报,2026,(4):1013-1024,12.基金项目
国家自然科学基金项目(32570110,32200087,32570127) （32570110,32200087,32570127）
广东省农业农村厅"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2024KJ19) （2024KJ19）
