园艺学报Issue(4)：1025-1037,13.DOI:10.16420/j.issn.0513-353x.2026-0001
枳丛枝菌根共生相关硝酸盐转运蛋白基因PtNPF5.2的功能鉴定
Functional Identification of a Nitrate Transport Gene PtNPF5.2 Related to Arbuscular Mycorrhizal Symbiosis in Poncirus trifoliata
摘要
Abstract
To investigate the molecular mechanism of nitrate(NO3-)transport via the arbuscular mycorrhiza(AM)pathway in citrus,this study used Poncirus trifoliata,a common citrus rootstock,to examine the expression pattern and subcellular localization of the NO3-transporter gene PtNPF5.2,as well as the role of its homologous genes in the AM symbiosis of the model plants Medicago truncatula and Solanum lycopersicum.The results showed that PtNPF5.2 was specifically induced by arbuscular mycorrhizal fungi(AMF),its promoter was activated in root cells containing arbuscules,and the encoded protein was localized to the plasma membrane.Interference or insertion mutation of the homologous gene MtNPF5.3 in Medicago truncatula did not affect AMF colonization.Similarly,CRISPR/Cas9 knockout of the homologous gene SlNPF5.2 in Solanum lycopersicum did not affect AMF colonization but led to a significant decrease in shoot NO3-content.In summary,PtNPF5.2 may be involved in regulating the NO3-allocation within the plant during AM symbiosis in Poncirus trifoliata,providing insights into the nitrogen transport mechanisms via the AM pathway in citrus.关键词
枳/丛枝菌根/硝酸盐转运蛋白/PtNPF5.2/氮转运/基因功能鉴定Key words
Poncirus trifoliata/arbuscular mycorrhiza/nitrate transport protein/PtNPF5.2/nitrogen transport/gene functional identification分类
农业科技引用本文复制引用
于淼,黄圣宇,潘志勇..枳丛枝菌根共生相关硝酸盐转运蛋白基因PtNPF5.2的功能鉴定[J].园艺学报,2026,(4):1025-1037,13.基金项目
国家重点研发计划项目(2024YFD2300800) （2024YFD2300800）
湖北省水果(柑橘、猕猴桃)产业技术体系项目(2025HBSTX4-08) （柑橘、猕猴桃）
湖北省柑橘产业链科技创新攻关项目(202501) （202501）
