园艺学报Issue(4)：1038-1056,19.DOI:10.16420/j.issn.0513-353x.2025-1229
丛枝菌根真菌与嗜松篮状菌LZ1协同缓解苹果连作障碍的机理研究
Mechanism of Synergistic Alleviation of Apple Replant Disease by Arbuscular Mycorrhizal Fungi and Talaromyces pinophilus LZ1
摘要
Abstract
This study aimed to develop a green and sustainable biocontrol strategy for apple replant disease(ARD)by exploring the alleviating effect of combined application of an arbuscular mycorrhizal fungi(Paraglomus sp.SW1)and an antagonistic Talaromyces pinophilus LZ1 on ARD.The results showed that the LZ1 strain isolated from the rhizosphere of chestnut not only significantly inhibited the growth of the main pathogenic Fusarium species causing ARD,but also increased the mycorrhizal colonization rate in apple seedlings grown in replant soil.Validation through greenhouse,pot,and field experiments revealed that the combined application of AMF and LZ1 significantly enhanced the biomass and mycorrhizal colonization in apple plants under replant soil.Compared with the replant control soil treatment without any microbial inoculants(CK-1),plant height,ground diameter,fresh weight,dry weight,and mycorrhizal colonization rate increased by 85.43%,88.20%,144.47%,145.40%,and 62.94%,respectively.The co-inoculation of AMF and LZ1 also significantly increased soil spore density and easily extractable glomalin-related soil protein(EE-GRSP)content,which were 59.87%and 46.98%higher than those in CK-1,respectively.Furthermore,the co-inoculation improved soil enzyme activities and optimized the soil microbial community structure.In summary,the synergistic application of AMF and LZ1 can provide a sustainable and eco-friendly strategy for alleviating ARD.关键词
苹果/连作障碍/丛枝菌根真菌/嗜松篮状菌/生物防治/土壤微生物群落Key words
apple/replant disease/arbuscular mycorrhizal fungi/Talaromyces pinophilus LZ1/biocontrol/soil microbial community分类
农业科技引用本文复制引用
高雅文,尹承苗,毛志泉,孙琳佼,刘宇松,孙文泰,徐明娜,宋乐芬,王雪梅,王功帅,王玫..丛枝菌根真菌与嗜松篮状菌LZ1协同缓解苹果连作障碍的机理研究[J].园艺学报,2026,(4):1038-1056,19.基金项目
国家自然科学基金项目(32502619) （32502619）
山东省重点研发项目(重大科技创新工程项目,2024CXGC010903) （重大科技创新工程项目,2024CXGC010903）
现代农业产业技术体系建设专项资助(CARS-27) （CARS-27）
