园艺学报Issue(4)：1057-1072,16.DOI:10.16420/j.issn.0513-353x.2025-1332
非靶向代谢组学揭示盐胁迫下两种菌根真菌介导的核桃根系激素响应研究
Non-Targeted Metabolomics Reveals Hormonal Responses in Walnut Roots Mediated by Two Mycorrhizal Fungi Under Salt Stress
摘要
Abstract
To investigate the response of mycorrhizal fungi to the physiological mechanisms of walnut seedling roots under salt stress,Funneliformis mosseae and Piriformospora indica were selected as the fungal strains,and potted greenhouse seedlings of Juglans regia'Yanyuanzao'were used as the plant material.Treatments included non-inoculation,single inoculation,and dual inoculation.Non-targeted metabolomics was employed to analyze the effects of the two mycorrhizal fungi and different inoculation methods on the content of plant endogenous hormones(jasmonic acid,salicylic acid,auxin,and abscisic acid)in the roots of walnut seedlings under short-term stress induced by 150 mmol·L-1 NaCl solution.The results showed that both mycorrhizal fungi could colonize the roots of walnut seedlings,with no significant difference in the colonization rate between the inoculation treatments.Under salt stress,mycorrhizal fungi significantly affected the number of metabolites in the roots of walnut seedlings.Among these,inoculation with P.indica(single or dual inoculation)resulted in the highest number of differential metabolites,with 505 and 527 species,respectively.Enrichment analysis of metabolic pathways revealed that under salt stress,F.mosseae influenced nitrogen metabolism and glutathione metabolism,P.indica activated the pentose phosphate pathway and pantothenate biosynthesis pathway,and the dual inoculation treatment regulated multiple pathways,including 2-oxocarboxylic acid metabolism and glycolysis.Under salt stress,the levels of endogenous hormones in walnut seedling roots changed,with a decrease in JA,SA,and IAA content and an increase in ABA content.Pre-inoculation with mycorrhizal fungi increased the levels of JA,SA,and IAA in the roots of walnut seedlings under salt stress.Among these,P.indica showed a greater ability to enhance JA and IAA levels,while dual inoculation significantly reduced ABA content.In summary,single and dual inoculations with F.mosseae and P.indica can colonize and form symbionts in the roots of walnut seedlings,promoting hormone synthesis and hormone signaling pathways,thereby enhancing the salt tolerance of walnut seedlings.The synergistic effect was more pronounced in the dual inoculation treatment.关键词
核桃/盐胁迫/菌根真菌/非靶向代谢组学/植物激素Key words
walnut/salt stress/mycorrhizal fungi/untargeted metabolomics/plant hormones分类
农业科技引用本文复制引用
吴承勖,聂瑞宁,姬新颖,唐佳佳,李敖,郑旭,张俊佩..非靶向代谢组学揭示盐胁迫下两种菌根真菌介导的核桃根系激素响应研究[J].园艺学报,2026,(4):1057-1072,16.基金项目
国家重点研发计划课题(2022YFD2200402) （2022YFD2200402）
