园艺学报2026，Vol.53Issue(5)：1274-1284,11.DOI:10.16420/j.issn.0513-353x.2025-0214
桑树MaNAC90的克隆及其在干旱胁迫下的功能分析
Cloning and Functional Analysis of Mulberry MaNAC90 Gene Under Drought Stress
摘要
Abstract
A NAC transcription factor gene MaNAC90 was cloned from Morus alba'Deguo 1'.The open reading frame of MaNAC90 was 732 bp,encoding 243 amino acids with molecular weight 27.436 kD and isoelectric point 5.32.MaNAC90 contained one NAC domain and belonged to TERN group,was localized in the nucleus and the cell membrane.Under simulated drought stress(200 mmol·L-1 mannitol),transgenic Arabidopsis expressing MaNAC90 exhibited a significant reduction in germination rate,a notable decrease in root length,an increased severity of seedling wilting,and significantly increased MDA content and electrolyte leakage,along with significantly decreased expression of RD22 and RD29B when compared to wild-type plants.These results preliminary indicated that MaNAC90 plays a negative regulatory role in response to drought stress.关键词
桑/果用/MaNAC90/干旱胁迫/功能分析Key words
mulberry/fruit use/MaNAC90/drought stress/function analysis分类
农业科技引用本文复制引用
黄仁维,李晓冬,邓红梅,杨晏,曾睿,杨财容..桑树MaNAC90的克隆及其在干旱胁迫下的功能分析[J].园艺学报,2026,53(5):1274-1284,11.基金项目
四川省科技创新创业苗子工程项目(2023JDRC0114) （2023JDRC0114）
成都师范学院高层次人才引进项目(2021YJRC2020-22) （2021YJRC2020-22）
特色园艺生物资源开发与利用四川省高校重点实验室项目(2023TSYY-02,TSYY202402Z) （2023TSYY-02,TSYY202402Z）
农村土地利用监测与评价重点实验室项目(NDZDS202409Z) （NDZDS202409Z）
大学生创新创业训练计划项目(S20221438914) （S20221438914）
