园艺学报2026，Vol.53Issue(5)：1343-1356,14.DOI:10.16420/j.issn.0513-353x.2025-0225
甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析
Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon
摘要
Abstract
To investigate the role of the TIFY gene family in the low temperature stress response of melon(Cucumis melo L.),genome-wide identification of TIFY family members was performed using bioinformatics approaches.Tissue-specific expression patterns and low temperature stress responsive profiles were further analyzed through transcriptome sequencing and quantitative reverse transcription polymerase chain reaction technology.The results demonstrated that a total of 15 TIFY family genes were identified in melon,classified into four subfamilies:JAZ,TIFY,ZIM,and PPD,and distributed across nine chromosomes.Tissue-specific expression analysis revealed that CmTIFY genes were expressed in various tissues.Among them,CmTIFY5A,CmTIFY5B,CmTIFY5C,CmTIFY5D and CmTIFY9B exhibited higher expression levels in flowers compared to other tissues.With fruit ripening,the gene expression level of CmTIFY5B gradually increased,whereas that of CmTIFY6B gradually decreased.CmTIFY5B and CmTIFY6B may be involved in the regulation of melon fruit ripening and quality formation.The expression levels of most CmTIFY genes were up regulated under low temperature stress,among which CmTIFY5B and CmTIFY5C were most significantly up regulated under low temperature.关键词
甜瓜/非生物胁迫/TIFY/基因家族/表达分析/低温胁迫Key words
melon/abiotic stress/TIFY/gene family/expression analysis/low temperature stress分类
农业科技引用本文复制引用
曹磊,李琼,毛文文,胡建斌,杜平,羊日令,周宇豪,侯娟,李翔,王盼乔,李丽丽,罗陈..甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析[J].园艺学报,2026,53(5):1343-1356,14.基金项目
国家自然科学基金项目(32302529,32472740) （32302529,32472740）
中国博士后科学基金第75批面上资助项目(2024M750813) （2024M750813）
河南省高等学校重点科研项目(24A210012) （24A210012）
