园艺学报2026，Vol.53Issue(5)：1371-1381,11.DOI:10.16420/j.issn.0513-353x.2025-0442
3种兜兰FAD2基因克隆及响应零上低温表达的差异分析
Cloning of FAD2 Genes in Three Paphiopedilum Species and Comparative Analysis of Their Expression in Responses to Chilling Stress
摘要
Abstract
Low temperature is a critical environmental factor limiting the survival and reproduction of Paphiopedilum species.Fatty acid desaturase 2(FAD2)plays a pivotal role in plant chilling tolerance by dynamically regulating the unsaturation of membrane lipids.Here,the sequence characteristics of FAD2 genes and their expression regulation under low-temperature conditions were systematically elucidated in three Paphiopedilum species with distinct ecological adaptations(P.purpuratum,P.dianthum,and P.armeniacum)through gene cloning,bioinformatics analysis,and qRT-PCR methods.The results revealed that the physicochemical parameters,spatial conformations,and transmembrane characteristics of the three FAD2 proteins were highly conserved.However,their subcellular localization predicted by WoLF PSORT exhibited marked species specificity:PaFAD2 from P.armeniacum showed predominant plastid localization,while PpFAD2 and PdFAD2 exhibited preferential accumulation in the plasma membrane/endoplasmic reticulum and diverse endomembrane systems,respectively.Under 4℃chilling stress,the PaFAD2 gene was rapidly activated at the early stress stage(24 h treatment group)and maintained high expression levels;whereas,PpFAD2 or PdFAD2 genes displayed delayed response or weak induction characteristics,respectively.Integrating biogeographical distribution patterns,it was hypothesized that the rapid transcriptional response of PaFAD2 in P.armeniacum might be evolutionarily associated with long-term adaptation to high-altitude habitats,whereas P.purpuratum and P.dianthum appeared to utilize differential FAD2 expression strategies to achieve an adaptive trade-off between cold resistance and metabolic efficiency.关键词
兜兰/FAD2/低温胁迫/基因克隆/表达特征Key words
Paphiopedilum/fatty acid desaturase 2/low-temperature stress/gene cloning/expression characteristics分类
农业科技引用本文复制引用
黄久妍,段晓芊,付月,常玮,丁勇..3种兜兰FAD2基因克隆及响应零上低温表达的差异分析[J].园艺学报,2026,53(5):1371-1381,11.基金项目
云南省中青年学术与技术带头人后备人才项目(202405AC350032) （202405AC350032）
云南省教育厅科学研究基金项目(2023Y0786) （2023Y0786）
中国科学院技术支撑人才项目(E4233811) （E4233811）
生物学省级学科建设经费资助项目(32004217) （32004217）
