中草药2026，Vol.57Issue(10)：3685-3692,8.DOI:10.7501/j.issn.0253-2670.2026.10.001
小酸浆中2种新的醉茄内酯及其抗肿瘤活性研究
Two new withanolides from Physalis minima and their antitumor activities
摘要
Abstract
Objective To investigate the withanolides from the aerial parts of Physalis minima and evaluate their in vitro anti-tumor activities against human colon cancer(HCT-116),triple-negative breast cancer(MDA-MB-231),and human erythroleukemia(HEL)cell lines.Methods The chemical constituents were isolated and purified using various chromatographic techniques.Their structures were elucidated through a comprehensive analysis of spectroscopic data,including infrared(IR),high-resolution mass spectrometry(HRMS),nuclear magnetic resonance(NMR),and electronic circular dichroism(ECD).The proliferation inhibitory activitiesof the isolated compounds against the above three cancer cell lines were evaluated using the MTT assay.Results Eight withanolides were isolated and identified from the aerial parts of P.minima,including two new compounds(1 and 2),which were characterized as(8R,9S,10R,13R,14R,16S,17R,20S,22R,24S)-14α,17α:16α,24α-diepoxy-13α,14β,22α-trihydroxy-1,15-dione-13,14-seco-ergosta-2,5-diene-18,20-olide(1)and(8R,9S,10R,13R,14R,16S,17R,20S,22R,24R,25R)-14α,17α:14,27-diepoxy-13α,22α-dihydroxy-1,15-dione-13,14-seco-ergosta-2,5-diene-18,20-olide-24-propionic acid(2).The known compounds were identified as 5 α-ethoxy-6 β-hydroxy-5,6-dihydrophysalin B(3),physalin VI(4),physaminin F(5),physagulide D(6),pubesenolide(7),and physaminilide I(8).The in vitro bioassay results demonstrated that three compounds(1,3,and 5)exhibited potent inhibitory activities against HCT-116 cells,with IC50 values ranging from(10.97±1.62)to(39.20±2.17)μmol/L.Compound 3 displayed an IC50 value of(19.36±1.03)μmol/L against MDA-MB-231 cells.Additionally,three compounds(1,3,and 4)showed inhibitory effects on HEL cells,with IC50 values ranging from(8.27±0.37)to(38.97±1.77)μmol/L.Conclusion Compounds 1 and 2 are new compounds.Compound 1 represents the first reported 22,26-seco-nor-physalin,named phyminate A,while compound 2 is the first 22,26-seco-physalin featuring a 24-propionic acid moiety and a C-14/C-27 ether bridge,named phyminate B.The inhibitory activities of compounds 1,3-5 against three tumor cell lines indicate their potential as anti-cancer candidates,justifying in-depth study.关键词
小酸浆/醉茄内酯/抗肿瘤活性/酸浆内酯A/酸浆内酯B/5α-乙氧基-6β-羟基-5,6-二氢酸浆素BKey words
Physalis minima L./withanolides/anti-tumor activity/phyminate A/phyminate B/5α-ethoxy-6β-hydroxy-5,6-dihydrophysalin B分类
医药卫生引用本文复制引用
许洪志,张锋,陶林岚,李亚男,苑春茂,金军,易平..小酸浆中2种新的醉茄内酯及其抗肿瘤活性研究[J].中草药,2026,57(10):3685-3692,8.基金项目
国家自然科学基金资助项目(32270413) （32270413）
国家自然科学基金资助项目(82360826) （82360826）
国家自然科学基金资助项目(82560693) （82560693）
