中草药2026，Vol.57Issue(10)：3700-3708,9.DOI:10.7501/j.issn.0253-2670.2026.10.003
南欧大戟的化学成分研究
Chemical constituents of Euphorbia peplus
摘要
Abstract
Objective To investigate the terpenoid constituents of fruit shells of Euphorbia peplus and evaluate their anti-inflammatory activities.Methods Various chromatographic techniques were applied to isolate terpenoids from the petroleum ether-soluble fraction of the ethanol extract of E.peplus fruit shells.The chemical structures of all isolates were elucidated by comprehensive spectroscopic analyses(1D/2D NMR,HRMS)combined with single-crystal X-ray diffraction.Anti-inflammatory activities were assessed by measuring the inhibition of nitric oxide(NO)production in lipopolysaccharide(LPS)-induced RAW264.7 macrophages.Results A total of 13 terpenoids were isolated from the petroleum ether fraction of E.peplus fruit shells,including four paraliane-type diterpenoids(1-4),one pepluane-type diterpenoid(5),three jatrophane-type diterpenoids(6-8),two ingenane-type diterpenoids(9 and 10),one abietane-type diterpenoid(11),one ursane-type triterpenoid(12),and one dinor-monocyclic diterpenoid(13).They were respectively identified as(2S,3S,4R,5R,6R,8S,12S,13S,15R)-8β-acetyl-paralianone D(1),(1R,2R,3S,4R,5R,6R,8S,12S,13S,14R,15R)-1,5,8,14-tetraacetoxy-3-benzoyloxy-15-hydroxy-9-oxo-paraliane(2),(2S,3S,4R,5R,6R,8S,12S,13S,14R,15R)-5,8,14-triacetoxy-3-benzoyloxy-15-hydroxy-9-oxo-parapliane(3),paralianone A(4),(2S,3S,4R,5R,6R,8R,9R,10R,12S,13S,14R,15R)-5,8,9,10,14-pentaacetoxy-3-benzoyloxy-15-hydroxypepluane(5),euphpepluone A(6),euphpepluone D(7),(2S,3S,4R,5R,7S,8R,13S,15R)-5α,7β,8α-triacetoxy-3β-benzoyloxy-15β-hydroxyjatropha-6(17),11E-diene-9,14-dione(8),20-deoxyingenol(9),20-O-(2'E,4'Z-decadienoyl)ingenol(10),helioscopinolide D(11),ursolic acid(12)and(3R,6R,7E,9E,11E)-3-hydroxy-13-apo-α-caroten-13-one(13).The structure of compound 1 was confirmed by single-crystal X-ray diffraction.In the anti-inflammatory activity screening,only compound 2 exhibited inhibitory activity against NO production,with an IC50 value of(37.6±8.2)μmol/L.Conclusion Compounds 1,2,10,and 13 were isolated from E.peplus for the first time.Compound 2 demonstrated moderate anti-inflammatory activity.This study enriches the terpenoid chemical diversity of terpenoids from E.peplus and provides an experimental basis for further research on its anti-inflammatory substances.关键词
大戟科/南欧大戟/二萜/抗炎/20-去氧巨大戟醇/熊果酸/(2S,3S,4R,5R,6R,8S,12S,13S,15R)-8β-acetyl-paralianone DKey words
Euphorbiaceae/Euphorbia peplus Linn./diterpenoids/anti-inflammatory/20-deoxyingenol/ursolic acid/8β-acetyl-paralianone D/(2S,3S,4R,5R,6R,8S,12S,13S,15R)-8β-acetyl-paralianone D分类
医药卫生引用本文复制引用
颜欣平,周兰婷,于静怡,李锦花,彭华勇,杨得坡..南欧大戟的化学成分研究[J].中草药,2026,57(10):3700-3708,9.基金项目
国家重点研发计划项目(2022YFD1600304) （2022YFD1600304）
湖南省教育厅项目(25C0336) （25C0336）
大学生创新创业训练计划项目(S202510531097) （S202510531097）
