中草药2026，Vol.57Issue(10)：3709-3719,11.DOI:10.7501/j.issn.0253-2670.2026.10.004
构树枝条的化学成分及其PEL细胞毒活性研究
Chemical constituents from branches of Broussonetia papyrifera and their cytotoxic activity against primary effusion lymphoma cells
摘要
Abstract
Objective To investigate the chemical constituents of the branches of Broussonetia papyrifera and screen for compounds with cytotoxic activity against primary effusion lymphoma(PEL)cells.Methods The compounds were isolated and purified by various chromatographic techniques including silica gel,RP-18,Sephadex LH-20,and MCI column chromatographies,as well as high-performance liquid chromatography(HPLC).Their structures were identified by spectroscopic methods.The cytotoxicity of the isolates against PEL cell lines(BC-3 and BCBL-1)was evaluated using the CCK-8 assay.The effects of active compounds on cell apoptosis and cell cycle distribution were analyzed by flow cytometry.Results Nineteen compounds were isolated and identified as broussoflavonol B(1),8-prenylquercetin-3-methyl ether(2),taxifolin(3),liquiritigenin(4),(+)-medioresinol(5),(+)-syringaresinol(6),dadahol B(7),diospyrosin(8),balanophonin(9),5-methoxybalanophonin(10),isokhellactone(11),nodakenetin(12),rutamarin(13),smyrindiol(14),marmesin(15),8-methoxymarmesin(16),7-demethylsuberosin(17),umbelliferone(18),and n-hexacosyl(E)-p-coumarate(19).Compounds 1,7 and 17 exhibited significant inhibitory activity against PEL cells.Among them,compound 7 showed half-maximal inhibitory concentration(IC50)values of 16.05 and 16.90 μmol/L against BC-3 and BCBL-1 cells,respectively.Further studies indicated that compound 7 could notably induce apoptosis and arrest cell cycle in PEL cells.Conclusion Compounds 8,17 and 19 were isolated from the Moraceae for the first time,while compounds 9-11 and 13 were obtained from the genus Broussonetia for the first time.Compound 7 exerts its anti-PEL activity by inducing cell apoptosis and blocking cell cycle progression.关键词
构树/dadahol B/diospyrosin/7-去甲基软木花椒素/反式对羟基桂皮酸二十六烷酯/原发性渗出性淋巴瘤/细胞毒活性Key words
Broussonetia papyrifera(L.)L'Hér.ex Vent/dadahol B/diospyrosin/7-demethylsuberosin/n-hexacosyl(E)-p-coumarate/primary effusion lymphoma/cytotoxic activity分类
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王佳乐,高丛西,刘兴行,王扣,周衍衡..构树枝条的化学成分及其PEL细胞毒活性研究[J].中草药,2026,57(10):3709-3719,11.基金项目
国家自然科学基金资助项目(82060367) （82060367）
云南省中青年学术技术带头人后备人才项目(202205AC160073) （202205AC160073）
陕西省高校优秀青年人才项目(GXYQ004) （GXYQ004）
延安大学研究生教育创新计划项目(YCX2024036) （YCX2024036）
